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大豆科学  2008 

野生大豆Δ′-吡咯琳-5-羧酸合成酶(P5CS)基因的克隆与序列分析

DOI: 10.11861/j.issn.1000-9841.2008.06.0915, PP. 915-920

Keywords: 野生大豆,Δ′-吡咯琳--羧酸合成酶,耐盐

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Abstract:

根据栽培大豆(Glycinemax)Δ′-吡咯琳-5-羧酸合成酶基因的全长序列设计引物,通过RT-PCR直接扩增的方法从野生大豆中克隆到了野生大豆(Glycinesoja)的同源基因,命名为GsP5CS。GsP5CS最大开放阅读框为2148bp,编码含有716个氨基酸残基、分子量为77.9kDa的蛋白,预测等电点6.21。亲疏水性分析显示,GsP5CS含有连续的亲水片断。序列分析显示,GsP5CS与栽培大豆(Glycinemax)、飞蛾豆(Vignaaconitifolia)、蒺藜苜蓿(Medicagotruncatula)、紫花苜蓿(Medicagosativa)、甘蓝型油菜(Brassicanapus)、拟南芥(Arabidopsisthaliana)、水稻(Oryzasativa)、普通小麦(Triticumaestivum)等植物的Δ′-吡咯琳-5-羧酸合成酶基因高度同源,序列相似性分别为94%、87%、87%、86%;75%、74%、72%。根据野生大豆与这8种植物的P5CS基因序列相似性所建立的进化树,显示与传统的分类地位基本一致。

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