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大豆科学  2008 

农杆菌介导法将SPS基因导入大豆的研究

DOI: 10.11861/j.issn.1000-9841.2008.03.0387, PP. 387-390

Keywords: 大豆胚尖,农杆菌介导,SPS基因

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Abstract:

长期以来,大豆遗传转化由于受基因型限制,受体系统不完善、实验结果重复性差等因素,使农杆菌介导的大豆遗传转化频率低下。农杆菌介导法多以子叶节为受体系统,但转化体极易形成嵌合体,导致后期筛选难度加大。基因枪方法的技术难度大、易引起基因沉默。以大豆胚尖为受体,采用农杆菌介导法将玉米蔗糖磷酸合成酶(SPS)基因导入大豆基因组中。由于胚尖具有较强的分生能力,再生细胞由转化细胞而来,极大地降低了生成嵌合体的可能性。卡那霉素抗性植株经PCR及Southern杂交等分子检测,证明目的基因已导入并整合到大豆基因组中。

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