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大豆科学  2008 

大豆M型细胞质雄性不育恢复基因SSR标记初步定位

DOI: 10.11861/j.issn.1000-9841.2008.03.0383, PP. 383-386

Keywords: 大豆,细胞质雄性不育,微卫星标记

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Abstract:

鉴于细胞质雄性不育(CytoplasmicMaleSterility,CMS)在作物杂种优势利用中的良好应用前景及分子标记对恢复系选育的应用价值,试验采用SSR分子标记对大豆CMS恢复系WR016恢复基因进行初步定位。利用197对大豆SSR引物对不育系W931A和恢复系WR016单株进行多态性筛选,52对引物在双亲间表现多态性,多态性频率26.39%,进一步利用这52对引物对10株不育系W931A和恢复系WR016单株、不育基因池、可育基因池和半不育基因池进行分析,表明位于A连锁群上的Satt276表现出良好的多态性。再利用合成的Satt276附近的引物进行分析,表明Satt684和Satt572也有良好的多态性。据此,可将大豆M-CMS恢复系WR016的恢复基因初步定位在A连锁群上。

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