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大豆科学  2008 

大豆地方品种遗传结构及其保存研究

DOI: 10.11861/j.issn.1000-9841.2008.03.0361, PP. 361-365

Keywords: 大豆,地方品种,SSR,纯度鉴定,遗传完整性,品种保存

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Abstract:

我国具有丰富的地方品种,但在大豆育种中利用率较低,这与对地方品种的保存和鉴定研究较少有关。通过利用全基因组SSR标记扫描的方法对地方品种羊眼豆和毛豆进行纯度鉴定,旨在为地方品种的纯化及遗传完整性的保存提供科学依据。结果表明:羊眼豆在502个SSR标记位点的多态性为44.8%,毛豆在405个SSR标记位点的多态性为41.2%;检测2个地方品种纯度所需的最少标记数和种子粒数分别为3个标记和4粒种子;根据遗传一致性分别对2个地方品种进行聚类,在相似系数为0.85时,均被分为3个组。因此,这2个地方品种纯度较低,根据遗传一致性分组情况,对两个地方品种的一组进行提纯后作为一个品种保存,而另外2个组则可分别作为2个不同的地方品种保存其遗传完整性。

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