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大豆科学  2008 

RNA干扰技术及在植物抗病研究中的应用

DOI: 10.11861/j.issn.1000-9841.2008.03.0521, PP. 521-526

Keywords: RNAi,作用机制,基因沉默,抗线虫,抗病毒病

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Abstract:

RNA干扰(RNAinterference,RNAi)技术是一项基因沉默新技术,自20世纪90年代被发现以来,现在已逐渐成为分子生物学和细胞生物学研究的有用工具之一。目前该技术已被广泛应用到植物功能基因组研究和植物抗病毒病研究中,在抗线虫病研究中应用较少。现综述了RNAi的分子机制、基因沉默的主要类型以及该技术在植物抗线虫病和抗病毒病研究中的应用。

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