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大豆科学  2008 

人粒细胞巨噬细胞集落刺激因子在大豆中表达的初步研究

DOI: 10.11861/j.issn.1000-9841.2008.02.0199, PP. 199-202

Keywords: hGM-CSF,茎尖,大豆

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Abstract:

构建了人粒细胞巨噬细胞集落刺激因子(hGM-CSF)的植物表达载体pCAMBIA3300-CSF-KD,转化大豆,获得转hGM-CSF基因的植株。hGM-CSF的植物表达载体pCAMBIA3300-CSF-KD,该因子末端被加上内质网驻留信号(KDEL),适合在植物中表达。用大豆合丰47的茎尖作为外植体,通过农杆菌介导的转化,获得17株T1转化株。PCR扩增验证转基因植株,检测到11株阳性植株。

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