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大豆科学  2008 

大豆质核互作雄性不育系NJCMS2A的育性恢复性遗传和育性恢复基因的SSR标记

DOI: 10.11861/j.issn.1000-9841.2008.02.0181, PP. 181-185

Keywords: 大豆,质核互作雄性不育,遗传,SSR标记

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Abstract:

利用组合NJCMS2A×中豆5号对大豆质核互作雄性不育系NJCMS2A的育性恢复性遗传进行研究,结果表明:NJCMS2A的育性恢复性遗传在不同年份间表现稳定,F1全部为可育株,F1∶2表现育性分离,可育株与不育株的分离比例经χ2测验符合15∶1,F2∶3中无育性分离的家系数∶分离比例符合(3∶1)的家系数∶分离比例符合(15∶1)的家系数经χ2测验符合7∶4∶4,说明在组合NJCMS2A×中豆5号中NJCMS2A的育性恢复性由两对显性重叠基因控制,验证了Bai和Gai的研究结果。随机选取组合NJCMS2A×中豆5号的一个F1∶2株行群体作为分子标记定位群体,采用903对大豆SSR引物对NJCMS2A育性恢复基因进行分子标记和定位,结果找到一个SSR标记Satt135与NJCMS2A育性恢复基因连锁,采用极大似然法计算重组率,利用Kosambi函数将重组率转化为遗传距离,得到Satt135与NJCMS2A育性恢复基因的遗传距离为11.47cM,参照Song等整合的大豆分子遗传图谱,将NJCMS2A育性恢复基因定位于D2连锁群上。

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