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大豆科学  2015 

大豆胚发育期酵母双杂文库的构建及与bHLH转录因子互作蛋白的筛选

DOI: 10.11861/j.issn.1000-9841.2015.05.0789, PP. 789-793

Keywords: 大豆,发育,酵母双杂,转录因子,cDNA文库

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Abstract:

构建了大豆幼胚生长期20~50dDSN(duplex-specificnulease)均一化cDNA酵母双杂文库,总克隆数为2.8×107cfu。为了筛选能与已知大豆bHLH转录因子GmbHLH3a相互结合的蛋白质,以转录因子GmbHLH3a为诱饵蛋白构建诱饵载体,GmbHLH3a诱饵载体无自激活活性,通过酵母双杂筛选到了两个能与诱饵蛋白相互作用的蛋白及其序列。

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