全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
大豆科学  2015 

大豆胚尖转化体系优化和cry1C*基因转化大豆的研究

DOI: 10.11861/j.issn.1000-9841.2015.05.0910

Keywords: 大豆(GlycinemaxL),胚尖,遗传转化,cryC

Full-Text   Cite this paper   Add to My Lib

Abstract:

以抗大豆食心虫品系东农8004的胚尖为外植体,通过对芽诱导和芽伸长培养基进行优化,建立8004的胚尖转化体系,利用该体系进行cry1C*基因的转化,对获得的抗性再生植株进行分子鉴定和抗虫性鉴定。结果表明:在芽诱导培养基中附加1.67mg.L-16-BA,丛生芽诱导率最高,达到80%,在芽伸长培养基中附加0.5mg.L-1GA3和0.1mg.L-1.IAA,芽伸长率最高为54%。共获得94株PPT(100μg.mL-1)抗性再生植株,PCR检测阳性14株,阳性率为14.89%。对4株T0代阳性植株进行大豆食心虫抗性鉴定,其中C-1植株的食心虫抗性明显高于野生型8004,说明在大豆中表达cry1C*基因能提高大豆对食心虫的抗性。

References

[1]  [1]王萍,张淑珍,李文滨,等.大豆不同基因型胚尖不定芽的诱导及对抗生素的敏感性[J].作物杂志, 2010(2): 50-53.(Wang P, Zhang S Z, Li W B, et al. Induction of adventitious shoots from embryonic tip of different soybean gene types and their sensibility to antibiotics [J].Crops, 2010(2): 50-53.)
[2]  [2]张东旭.大豆胚尖再生体系的建立及E-2基因遗传转化[D].石家庄: 河北农业大学, 2008.(Zhang D X. Establishment of an efficient plant regeneration system via embryonic tips of soybean and Agrobacterium-mediated E-2 gene transformation using the embryonic tips explants[D]Shijiazhuang: Agricultural University of Hebei, 2008.)
[3]  [3]林树柱,曹越平,卫志明,等.6-BA诱导大豆子叶节和茎尖出芽的研究[J]. 上海交通大学学报, 2005, 23(2): 138-142.(Lin S Z, Cao Y P, Wei Z M et al.Studies on shoots induced by 6-BA from cotyledonary nodes and embryonic tips of soybean[J]. Journal of Shanghai Jiaotong University, 2005, 23(2): 138-142.)
[4]  [4]李海燕,武小霞,刘森,等.大豆子叶节、胚尖再生植株的研究[J].大豆科学, 2007, 26(5): 709-712.(L H Y, Wu X X, Liu M et al.Plant regeneration from cotyldeonary nodes and embryonic tips of soybean[J]. Soybean Science, 2007, 26(5): 709-712.)
[5]  [5]马晓红,姚陆铭,武天龙.大豆整个子叶节外植体再生体系的建立及与子叶节、胚尖再生体系的比较[J].大豆科学, 2008, 27(3): 373-378.(Ma X H, Yao L M, Wu T L.High frequency plant regeneration from whole cotyledonary node explants and comparison with cotyledonary node and embryonic tip regeneration system in soybean[J]. Soybean Science, 2008, 27(3): 373-378.)
[6]  [6]张东旭,张洁,商蕾,等.大豆胚尖再生体系的研究[J]. 河北农业大学学报, 200, 31(4): 7-13.(Zhang D X, Zhang J, Shang L et al.Study on the regeneration system of soybean embryonic tips [J].Journal of Agricultural University of Hebei, 2008, 30(4): 7-13-)
[7]  [7]王继安,罗秋香.大豆食心虫抗性品种鉴定及抗性性状分析[J].中国油料作物学报, 2001, 23(2): 57-59.(Wang J A, Luo Q X.Evaluation of soybean pod borer resistance and analysis of resistance characteristics in soybean[J]. Chinese Journal of Oil Crop Sciences, 2001, 23(2): 57-59.)
[8]  [8]刘海坤,卫志明.一种大豆成熟种子的消毒方法[J].植物生理学通讯, 2002, 38(3): 260-261.(Liu H K, Wei Z M. A method for sterilizing mature seeds of soybean[J].Plant Physiology Journal, 2002, 38(3): 260-261.)
[9]  [9]王伟,王罡,季静,等.大豆胚尖再生体系的优化及与子叶节再生体系的比较[J].大豆科学, 2012, 31(3): 353-357.(Wang W, Wang G, Ji J, et al. Optimization of embryonic tip regeneration system and comparison with cotyledonary node regeneration system in soybean[J]. Soybean Science,2012, 31(3): 352-356.)
[10]  [10]王帆.Gy1-Gy7嵌合基因表达载体转化烟草的研究及大豆胚尖法再生体系的改良[D].上海: 上海师范大学, 2009.(Wang F. Study on transformation of Gy1-Gy7 chimeric gene plant express vectors to tobacco and the improvement of embryonic tips regeneration system of soybean[D]. Shanghai: Shanghai Normal University, 2009.)
[11]  [11]张洁,商蕾,郑文永,等.大豆胚尖遗传转化体系优化及抗逆基因GmPK转化研究[J]. 植物遗传资源学报, 2012, 13(2): 271-277.(Zheng J, Shang L, Zheng W Y, et al.Optimizing genetic transformation system of soybean embryonic tips and transformation of stress resistance gene GmPK[J].Journal of Plant Genetic Resources, 2012, 13(2): 271-277.)
[12]  [12]邓向阳,卫志明.大豆转化技术[J].植物生理学通讯, 1998, 34(5): 381-387.(Deng X Y, Wei Z M. Technique of transformation of soybean[J] Plant Physiology Journal, 1998, 34(5): 381-387.)
[13]  [13]赖冰冰,韩阳,李春风,等.大豆子叶节植株再生体系的研究[J].大豆科学, 2011, 30(2): 303-305.(Lai B B, Han Y, Li C F, et al.Research on cotyledonary nodes regeneration system of soybean[J].Soybean Science, 2011, 30(2): 303-305.)
[14]  [14]刘金华,王丕武,武丽敏,等.大豆子叶节丛生芽的诱导[J].吉林农业大学学报, 2001, 23(4): 15-17.(Liu J H, Wang P W, Wu L M, et al. The induction of multiple shoots via cotyledon nodes of soybean[J]. Journal of Jilin Agricultural University, 2001, 23(4): 15-17.)
[15]  [15]宋波,张兴国,苏承刚,等.大豆胚芽尖再生体系的建立[J]西南师范大学学报, 2010, 35(1): 128-131.(Song B, Zhang X G, Su C G, et al.Establishment of an embryonic tip regeneration system for soybeans[J]. Journal of Southwest China Normal University, 2010, 35(1): 128-131.)
[16]  [16]唐薇,张启发.Bt抗虫水稻的培育[D].武汉: 华中农业大学, 2006.(Tang W, Zhang Q F. Production of insect-resistant rice through Bt genes transformation[D].Wuhan: Huazhong Agricultural University,2006.)
[17]  [17]Xu X L,Han Y et al. Field evaluation of effects of transgenic cry1Ab/cry1Ac, cry1C and cry2A rice on Cnaphalocrocis medinalis and its arthropod predators[J] Science China Life Science, 2011, 54(11): 1019-1028.
[18]  [18]Han Y, Cai W L. Field evaluation of effects of transgenic cry1Ab/cry1Ac, cry1C and cry2A rice on Cnaphalocrocis medinalis and its arthropod predators[J]. Science China Life Science, 2011, 54(11): 1019-1028.
[19]  [19]Fujimoto H, Itoh K, Yamamoto M, et al. Insect resistant rice generated by introduction of a modified δ-endotoxin gene of Bacillus thuringiensis[J].Nature Biotechnology, 1993, 11(10): 1151-1155.
[20]  [20]Tang W, Chen H, Xu C, et al. Development of insect-resistant transgenic indica rice with a synthetic cry1C* gene[J] Molecular Breeding, 2006, 18(1): 1-10.
[21]  [21]Chen H, Tang W, Xu C, et al. Transgenic indica rice plants harboring a synthetic cry2A* gene of Bacillus thuringiensis exhibit enhanced resistance against lepidopteran rice pests[J].Theoretical and applied genetics, 2005, 111(7): 1330-1337.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133