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大豆科学  2015 

Biolog-ECO解析淡豆豉发酵过程中微生物群落碳代谢特征

DOI: 10.11861/j.issn.1000-9841.2015.05.0889, PP. 889-893

Keywords: 淡豆豉,微生物,Biolog生态微平板,代谢活性,多样性

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Abstract:

微生物是淡豆豉发酵的核心,研究淡豆豉发酵炮制过程中微生物群落碳代谢情况,对于揭示淡豆豉炮制机理意义重大。采用Biolog生态微平板研究了不同发酵时间点淡豆豉样品的碳代谢特征。结果表明:发酵第6天样品的AWCD值在整个培养过程中明显高于其它样品,发酵第1天,再闷9和15d样品的AWCD值曲线差异不明显;多样性分析显示,各样品间微生物Shannon-Wiener指数H′值相近,而第6天样品的Mclntosh指数U值明显高于其它发酵时间点的样品;各样品微生物的最佳碳源种类相近,主要利用了β-甲基-D-葡萄糖苷、N-乙酰-D-葡萄糖胺、D-甘露醇、葡萄糖-1-磷酸盐、D-半乳糖醛酸、吐温40等碳源,对γ-羟基丁酸、α-丁酮酸、L-精氨酸、L-α-甘油、i-赤藻糖醇等利用较少。研究表明,发酵第6天微生物群落的碳代谢活性最高;各样品微生物的丰富度相近,第6天样品微生物的均匀度与其它样品差别较大;整个发酵过程微生物的最佳碳源种类相近,主要利用了糖类及其衍生物,而对氨基酸、脂肪酸、脂类利用较少。ECO板能真实反映出淡豆豉发酵中微生物对碳源利用的整体代谢情况,但也有其局限性。

References

[1]  [1]李娜,黄庆柏.淡豆豉中的异黄酮成分及药理作用与临床应用[J].中国现代中药,2008,10(7):18-19. (Li N, Huang Q B. The pharmacological effects and clinial application of isoflavone contents in Semen sojae praeparatum[J].Modern Chinese Medicine,2008,10(7):18-19.)
[2]  [2]周庆伍,李红歌,李安军,等.Biolog Eco解析不同产地大曲微生物群落功能多样性特征[J].食品与发酵科技,2014,50(3):53-56.(Zhou Q W, Li H G, Li A J, et al. Analysis of microbial community functional diversity in Daqu from different regions using Biolog-Eco method[J]. Food and Fermentation Technology, 2014, 50(3): 53-56.)
[3]  [3]李刚,梁永红,龙凯,等.再闷过程影响淡豆豉炮制工艺研究[J].中草药,2014,45(8):1083-1088.(Li G, Liang Y H, Long K, et al. Effect of secondary fermentation on processing technology for fermented soybeans[J]. Chinese Traditional Herbal Drugs, 2014, 45(8): 1083-1088.)
[4]  [4]金剑,王光华,陈雪丽,等.Biolog-ECO解析不同大豆基因型R1期根际微生物群落功能多样性特征[J].大豆科学,2007,26(4):565-570. (Jin J, Wang G H, Chen X L, et al. Analysis of microbial community functional biology diversity in rhizosphere of different soybean genotypes at R1 stage using Biolog-ECO method[J].Soybean Science,2007,26(4):565-570.)
[5]  [5]郑丽萍,龙涛,林玉锁,等.Biolog-ECO解析有机氯农药污染场地土壤微生物群落功能多样性特征[J].应用与环境生物学报,2013,19(5):759-765.(Zheng L P, Long T, Lin Y S, et al. Biolog-ECO analysis of microbial community functional diversity in organochlorine contaminated soil[J]. Chinese Journal of Applied and Environmental Biology, 2013, 19(5): 759-765.)
[6]  [6]Classen A T, Boyle S I, Haskins K E, et al. Community-level physiological profiles of bacteria and fungi: Plate type and incubation temperature influences on contrasting soils [J]. FEMS Microbiology Ecology, 2003, 44 (3): 319-328.
[7]  [7]罗倩,黄宝灵,唐治喜,等新疆盐渍土3种植被类型土壤微生物碳源利用[J].应用与环境生物学报,2013,19 (1):96-104. (Luo Q, Huang B L, Tang Z X, et al. Carbon source utilization of microbes in saline soil of three vegetation types in Xinjiang, China[J]. Chinese Journal of Applied and Environmental Biology, 2013, 19 (1): 96-104.)
[8]  [8]Choi K, Dobbs F C. Comparison of two kinds of Biolog microplates(GN and ECO) in their ability to disiguish among aquatic microbialcommunities [J]. Journal of Microbiological Methods, 1999, 36: 203-213.
[9]  [9]Magurran A E. Ecological diversity and its measurement [M]. New Jersey:Princeton University Press, 1988: 43-47.
[10]  [10]贾夏,董岁明,周春娟.微生物生态研究中Biolog Eco 微平板培养时间对分析结果的影响[J].应用基础与工程科学学报,2013,21(1):10-18. (Dong X, Dong S M, Zhou C J. The effect of incubation time of Biolog-ECO Plate on the analysis result in the study of microbial ecology [J].Journal of Basic Science and Engineering,2013,21(1):10-18.)
[11]  [11]张燕燕,曲来叶,陈利顶.Biolog Ecoplate .TM.实验信息提取方法改进[J].微生物学通报,2009,36(7):1083-1091.(Zhang Y Y, Qu L Y, Chen L D. An amendment on information extraction of Biolog ECOplate-TM[J]. Microbiology China, 2009,36(7):1083-1091)

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