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大豆科学  2015 

转Na+/H+逆向转运蛋白基因(AlNHX1)大豆纯合系筛选及生理分析

DOI: 10.11861/j.issn.1000-9841.2015.03.0378, PP. 378-383

Keywords: 大豆(GiycinemaxL),钠氢逆向转运蛋白基因(AlNHX),转基因植株,耐盐能力

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Abstract:

为了获得转Na+/H+逆向转运蛋白基因(AlNHX1)大豆纯合株系并验证其盐胁迫条件下生理反应,对转AlNHX1基因T3代材料进行了PCR筛选和RT-PCR鉴定,并在水培条件下研究了纯合系植株耐盐性、K+/Na+比值、丙二醛(MDA)、过氧化氢(H2O2)含量和相关抗氧化酶活性变化。结果显示经PCR和RT-PCR鉴定,野生型植株没有出现目的条带,而转基因株系有相应的目的条带,表明AlNHX1基因已经整合到大豆基因组中并正常转录。在盐胁迫条件下,转基因各株系成活率明显高于野生型对照,地上部干重和根部干重较野生型显著增加,达到了显著差异水平(P<0.05)。此外,在盐胁迫条件下,转基因幼苗能维持根和叶中较高的K+/Na+比值,同时伴随着植株体内MDA和H2O2含量的下降以及超氧化物歧化酶(SOD)和过氧化酶(POD)的增加。本研究结果表明供试外源AlNHX1基因在大豆高代材料中超表达,提高了转基因大豆耐盐能力,可为进一步培育适应盐渍化土壤环境下生长的大豆新品种提供重要的种质资源。

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