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大豆科学  2015 

叶酸代谢途径folE基因表达载体的构建及在大豆中的转化

DOI: 10.11861/j.issn.1000-9841.2015.02.0224, PP. 224-227

Keywords: 大豆,叶酸,folE,转基因

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Abstract:

folE基因是高叶酸调控基因,来源于细菌,已证明在西红柿等植物中,提高了叶酸含量。本试验采用农杆菌介导的大豆子叶节法,将folE基因在大豆受体材料Williams82中表达。共侵染了200个外植体,得到34株转化苗。通过对转化苗进行除草剂(135mg?L-1)涂抹鉴定,获得14株除草剂抗性转化苗。PCR检测结果显示其中12株为阳性。Southern杂交结果表明,folE全长cDNA已成功插入到大豆基因组中,并得到2个单拷贝株系。

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