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大豆科学  2015 

大豆子叶节遗传转化体系优化及抗逆基因AtNHX5的转化研究

DOI: 10.11861/j.issn.1000-9841.2015.02.0205, PP. 205-211

Keywords: 大豆,子叶节,AtNHX基因,遗传转化,除草剂

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Abstract:

以河北省优良大豆品种冀豆15、五星2号和NF-58的子叶节为受体材料,利用农杆菌介导法进行遗传转化,探讨了影响农杆菌侵染后子叶节不定芽诱导的因素。结果表明:以萌发6d、4℃处理24h的子叶节为外植体,农杆菌侵染后经超声波处理30s、共培养基中添加20mg?L-1硝酸银,能够提高子叶节丛生芽诱导率,转化植株经草铵膦筛选以及PCR检测,T0代转化率达0.97%。利用该体系对大豆品种五星2号进行AtNHX5基因的遗传转化,获得3株T0代RT-PCR检测阳性植株,且2-1号T1代阳性植株检测具有一定耐盐性,初步证明获得了转AtNHX5基因的大豆新材料。

References

[1]  [1]Stacey G, Vodkin L, Parrott W A, et al.National science foundation-sponsored workshop report.Draft plan for soybean genomics[J] Plant Physiology, 2004, 135 (1) : 59-70
[2]  [2]Cho. H J, Widholm J M.Agrobacterium tumefaciens-mediated transformation of the legume Astragalus sinicus using kanamycin resistance selection and green fluorescent protein expression[J] Plant Cell, Tissue and Organ Culture, 2002, 69 (3) : 251-258
[3]  [3]Meurer C A, Dinkins R D, Collins G B. Factors affecting soybean cotyledonary node transformation[J] Plant Cell Reports, 1998, 18: 180-186
[4]  [4]Trick H N, Finer J J. Sonication-assisted Agrobacterium-mediated transformation of soybean (Glycine max[L]Merrill) embryogenic suspension culture tissue[J] Plant Cell Report, 1998, 17 : 482-488
[5]  [5]李海燕, 刘淼, 武小霞, 等.大豆转化过程中的褐化现象研究[J] 作物杂志, 2010(1): 33-35(Li H Y, Liu M, Wu X X, et al. Study on the browning during soybean transformation[J]Crops, 2010(1):33-35
[6]  [6]Blumwald E. Sodium transport and salt tolerance in plants[J]Current Opinion in Cell Biology, 2000, 12 (4) : 431-434
[7]  [7]Li T X, Zhang Y, Zhang H X, et al.Stable expression of Arabidopsis vacuolar Na+/H+?antiporter gene AtNHX1, and salt tolerance in transgenic soybean for over six generations[J]Chinese Science Bulletin, 2010, 55 (12): 1127-1134
[8]  [8]Trieu A T, Burleigh S H.Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium[J] The Plant Journal, 2000, 22 (6): 531-541.
[9]  [9]Liu H, Wang Q, Yu M, et al.Transgenic salt-tolerant sugar beet constitutively expressing an Arabidopsis thaliana vacuolar Na+/H+?antiporter gene, AtNHX3, accumulates moresoluble sugar but less salt in storage roots[J] Plant Cell Environment, 2008, 31 (9) : 1325-1334
[10]  [10]Li M, Li Y, Li H Q, et al.Over expression of AtNHX5 improves tolerance to both salt and drought stress in Broussonetia papyrifera(L)Vent[J] Tree Physiology, 2011, 31(3): 349-357
[11]  [11]Shuji Y, Francisco J, Beatriz C, et al. Differential expression and function of Arabidopsis thaliana NHX Na+/H+?antiporters in the salt stress response[J] The Plant Journal, 2002, 30 (5) : 529-539
[12]  [12]Olhoft P M, Flagel L E, Donovan C M.Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method[J] Planta, 2003, 216: 723-735
[13]  [13]Tang W, Ross W, Ron S.Advances on genetic transforrmation in conifers[J]Developmental and Reproductive Biology, 2001, 10: 77-85
[14]  [14]Devi M K, Sakthivela G, Giridhar P. Protocol for augmented shoot organogenesis in selected variety of soybean (Glycine max[L] Merr)[J] Journal of Experimental Biology, 2012, 50(10):729-734
[15]  [15]Jefferson R A.Assaying chimeric in genes in plants : the GUS gene fusion system[J]Plant Molecular Biology, 1987, 5(4) : 387-405.
[16]  [16]于娅, 刘莉莎, 赵永钦, 等.影响花椰菜农杆菌介导转化因素的研究[J]植物遗传资源学报, 2010, 11(3) : 320-325(Yu Y, Liu L S, Zhao Y Q, et al.A study on the factors affecting the Agrobacterium-mediated transformation of cauliflower(Brassica oleracea var-botrytis) [J]Journal of Plant Genetic Resources, 2010, 11(3):320-325.
[17]  [17]李丹丹,张洁, 刘娜,等.农杆菌介导的大豆子叶节非组织培养遗传转化体系优化[J]植物遗传资源学报, 2012, 13, (5): 789-797(Li D D, Zhang J, Liu N, et al.Optimization of the Agrobacterium-mediated genetic transformation system of soybean cotyledonary node with non tissue-culture[J]Journal of Plant Genetic Resources, 2012, 13(5):789-797
[18]  [18]王关林, 方宏筠.植物基因工程[M]北京: 科学出版社, 2002 : 744(Wang G L, Fang H J. Plant gene engineering[M]Beijing: Science Press, 2002:744.
[19]  [19]Santarém E R, Trick H N, Finer J J. Sonication-assisted Agrobacterium-mediated transformation of soybean immature cotyledons: Optimization of transient expression[J] Plant Cell Report, 1998, 17: 752-759
[20]  [20]Trick H N, Finer J J.SAAT:Sonication-assisted Agrobacterium-mediated transformation[J]Transgenic Research, 1997, 6(5):329-336
[21]  [21]Hussain S S, Hussain T.Sonication-assisted Agrobacterium-mediated transformation (SAAT):An alternative method for cotton transformation[J]Pakistan Journal of Botany, 2007, 39(1): 223-230
[22]  [22]Febres V J, Costa M G. High-efficiency Agrobacterium-mediated transformation of citrus ?via-sonication and vacuum infiltration[J]Plant Cell Report, 2009, 28 (3): 387-395.
[23]  [23]Flores Solís J I, Mlejnek P, Studená K S.Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L[J] Plant Soil Environment, 2003,49 (6): 255-260
[24]  [24]Joersbo M, Brunstedt J.Sonication: A new method for gene transfer to plants[J]Physiologia Plantarum, 1992, 85: 230-234
[25]  [25]刘莉莉, 刘丹, 丛郁, 等. 超声波辅助对农杆菌介导新红星苹果遗传转化GUS基因瞬间表达的影响[J]江苏农业学报, 2008, 24 (2): 213-215(Liu L L, Liu D, Cong Y, et al.Effects of sonication assisted Agrobacterium-mediated transformation instantaneous expression of GUS gene in Xinhongxing apple during genetic transformation[J]Jiangsu Journal of Agricultural Sciences, 2008, 24(2):213-215
[26]  [26]王昌陵, 赵军, 李英慧, 等. 转录因子ABP9转化大豆[Glycine max(L) Merrill]及遗传转化条件优化[J]中国农业科学, 2008, 41 (7): 1908-1916(Wang C L, Zhao J, Li Y H, et al.Transforming transcription factor ABP9 into soybean[Glycine max(L) Merrill] and optimization of the transformation system[J] Scientia Agricultura Sinica, 2008, 41(7):1908-1916.
[27]  [27]刘金华, 王丕武, 吴丽敏,等.脯氨酸、硝酸银对农杆菌转化大豆的影响[J]大豆科学, 2003, 22 (1): 36-39(Liu J H, Wang P W, Wu L M, et al.Effection of proline and AgNO3?on Agrobacterium-mediated transformation of soybean[J]Soybean Science, 2003, 22(1):36-39)
[28]  [28]罗丽华, 陈建华, 苏冬梅, 等.板栗组培过程中褐变研究初探[J]经济林研究, 2003, 21(4): 30-31(Luo L H, Chen J H, Su D M, et al.Primary study on the browning of chestnut in plant tissue culture[J]Economic Forest Researches, 2003, 21(4):30-31
[29]  [29]Meyer P.Repeat induced gene silence:Common mechanisms in plantand fungi[J]Biology Chemistry Hoppe Seyler, 1996, 377 (2): 87-95

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