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大豆科学  2015 

AMP对铝胁迫诱导丹波黑大豆柠檬酸分泌及其铝耐受性的影响

DOI: 10.11861/j.issn.1000-9841.2015.01.0082, PP. 82-86

Keywords: 铝胁迫,丹波黑大豆,铝诱导的柠檬酸分泌,--蛋白,质膜H+ATP酶

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Abstract:

为了考察AMP(Adenosine5’monophosphate)对铝耐受型丹波黑大豆(RB)柠檬酸的分泌及铝抗性的影响,在50mmol?L-1铝溶液中添加100mmol?L-1?AMP共处理RB,结果表明RB根的相对生长率显著小于单独用50mmol?L-1铝胁迫处理的植株。免疫共沉淀分析显示铝胁迫下AMP的存在降低RB根中14-3-3蛋白与磷酸化质膜H+ATP酶的相互作用,使根尖质膜H+ATP酶活性降低约1倍,氢泵活性和H+分泌能力显著降低,根柠檬酸的分泌量减少约1倍。说明铝胁迫下AMP通过抑制14-3-3蛋白和质膜H?+ATP酶的互作来减少RB根尖的氢泵活性和柠檬酸分泌作用,从而降低RB根的相对生长率及其对铝胁迫的耐受性。

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