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大豆科学  2013 

卡那霉素和草铵膦对不同基因型大豆胚尖不定芽诱导的影响

DOI: 10.3969/j.issn.1000-9841.2013.01.032, PP. 136-138

Keywords: 大豆,胚尖,不定芽,卡那霉素,草铵膦

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Abstract:

以合丰35、黑农44和吉林35的胚尖为外植体,分别考察了不同浓度的卡那霉素和草铵膦对不同基因型大豆胚尖不定芽诱导的影响。结果表明,不定芽诱导率合丰35和吉林35在卡那霉素100mg.L-1时与对照差异不显著,黑农44在100mg.L-1处理时显著低于对照;芽数吉林35在卡那霉素100mg.L-1时显著低于对照并高于其他浓度,而合丰35与黑农44在100mg.L-1时芽数与对照差异不显著;3种基因型大豆胚尖不定芽伸长均在卡那霉素浓度为100mg.L-1时受到显著抑制。草铵膦浓度在0.2~1.2mg.L-1时对合丰35和吉林35的芽数没有影响,但黑农44在1.0mg.L-1时芽数开始显著低于对照;芽长合丰35、黑农44和吉林35分别在0.6、0.2和0.2mg.L-1时显著低于对照。因此,合丰35、黑农44和吉林35的适宜卡那霉素的筛选浓度为100mg.L-1,草铵膦浓度分别为0.6、0.2和0.2mg.L-1。

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