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大豆科学  2013 

抗草甘膦野生大豆筛选及其抗性生理机制研究

DOI: 10.3969/j.issn.1000-9841.2013.01.018, PP. 76-83

Keywords: 野生大豆,草甘膦,莽草酸,叶绿素含量,谷胱甘肽-S-转移酶

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Abstract:

利用田间试验对67份野生大豆材料进行草甘膦抗性筛选,结果在草甘膦有效剂量为1.23kg.hm-2条件下,不同野生大豆对草甘膦抗性存在明显差异,其中ZYD0685和ZYD2405的存活率最高,分别为87%和83%;7份材料的存活率为2.7%~38%;58份材料死亡。以抗性材料ZYD0685和敏感材料ZYD0790进行生理指标研究,结果在未经草甘膦处理时,二者的叶绿素含量、莽草酸含量及谷胱甘肽-S-转移酶(GSTs)活性基本相同。在草甘膦不同剂量处理下,抗性材料ZYD0685叶绿素和莽草酸含量没有明显变化,GSTs活性明显增加;而敏感材料ZYD0790莽草酸含量明显升高,叶绿素含量明显降低,GSTs活性变化幅度较小。因此,植物体内莽草酸积累量较少是抗草甘膦的主要生理反应。

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