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大豆科学  2013 

大豆不定胚悬浮培养基因型筛选及基因枪遗传转化的研究

DOI: 10.3969/j.issn.1000-9841.2013.01.009, PP. 38-45

Keywords: 大豆,不定胚悬浮培养,基因枪,基因型筛选

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Abstract:

对32个不同基因型大豆的幼胚进行不定胚诱导,30d时统计分析初生胚诱导率、次生胚诱导率和诱导效率,经综合比较得出适合体细胞诱导不定胚的4种基因型为L155、垦丰16、绥农25和吉育91。然后以不定胚诱导率较高的L155为材料建立大豆悬浮培养不定胚繁殖体系,并用基因枪轰击的方法将GUS表达盒片段转入不定胚细胞团。X-gluc染色后显微观察到基因蓝色产物,说明GUS基因通过微弹轰击法导入大豆不定胚细胞并表达。

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