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大豆科学  2013 

RAV基因对拟南芥和大豆不定芽再生的影响

DOI: 10.3969/j.issn.1000-9841.2013.01.006, PP. 23-32

Keywords: 大豆,GmRAV,拟南芥,不定芽再生,细胞分裂素

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Abstract:

RAV基因是具有AP2/ERF结构域的转录因子家族成员之一,以拟南芥rav突变体和GmRAV干涉(GmRAV-i)大豆的不同外植体为材料进行愈伤组织诱导,分别在芽诱导培养基中加入不同浓度的外源细胞分裂素2-ip和6-BA,考察平均不定芽数和不定芽再生频率。结果表明:与野生型材料相比,拟南芥rav突变体和GmRAV-i大豆在适宜的2-ip和6-BA浓度下平均不定芽数和不定芽再生频率均有所降低。证明RAV基因同系物在不同植物间具有功能保守性,并且在细胞分裂素存在下促进植物不定芽的再生。

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