全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
大豆科学  2014 

耐高氮优良大豆根瘤菌株的筛选与鉴定

DOI: 10.11861/j.issn.1000-9841.2014.06.0861, PP. 861-865

Keywords: 筛选,大豆根瘤菌,耐氮,优良菌株

Full-Text   Cite this paper   Add to My Lib

Abstract:

采用高氮平板与蛭石盆栽相结合的方法,以黑龙江省农业科学院1979年设立的黑土长期定位试验7种不同施肥处理中分离到的254株大豆根瘤菌为材料,筛选耐高氮的优良大豆根瘤菌。高氮平板结果显示:随着尿素浓度的增加,可生长的菌株数量逐渐减少,其中严重抑制菌株生长的尿素浓度为5g.L-1,该浓度条件下只有11株菌能够生长,均来自连续施用氮肥的处理。进一步采用模拟高氮环境蛭石盆栽的方法,将能在5g.L-1尿素条件下生长的11株菌进行复筛,以植株干重、植株全氮量、根瘤干重和数量作为评价指标,获得1株在高氮条件下具有结瘤固氮能力的菌株5841。经16SrDNA序列系统发育分析,初步确定该菌株属于日本慢生大豆根瘤菌(Brandyrhizobiumjaponicum)。

References

[1]  [1]李俊,沈德龙,林先贵.农业微生物研究与产业化进展[M].北京:科学出版社,2011:289.(Li J,Shen D L,Lin X G.Agricultural microbial research and industrialization progress[M].Beijing:Science Press,2011:289.)
[2]  葛城.微生物肥料生产及其产业化[M].北京:化学工业出版社,2007.(Ge C.Microbial fertilizer production and industrialization[C].Beijing:Chemical Industry Press,2007.)
[3]  王卫卫.东北地区大豆根瘤菌遗传多样性和适中低温菌株筛选及其生长机理研究[D].北京:中国农业科学院,2013.(Wang W W.Genetic diversity,screening of medium and low temperature-adapting strains and growth mechanism study of soybean rhizobia isolated from northeast regions[D].Beijing:Chinese Academy of Agricultural Sciences,2013.)
[4]  Rigaud J,Puppo A.Indole-3-acetic acid catabolism by soybean bacteroids[J].Journal of General Microbiologyl,1975,88:223-228.
[5]  ?Vincent J M.A manual for the practical study of the root-nodule bacteria[M].London:Blackwell Scientific Publications,1970.
[6]  Terefework Z,Kaijalainen S,Lindstrom K.AFLP Fingerprinting as a tool to study the genetic diversity of Rhizobium galegae isolated from Galega orientalis and Galega officinalis[J].Journal of Biotechnology,2001,41:169-180.
[7]  Yue L C,Jing Y W,En T W,et al.Bradyrhizobium lablabi sp.nov.isolated from effective nodules of Lablab purpureus and Arachis hypogaea[J].International Journal of Systematic and Evolutionary Microbiology,2010,61(10):2496-2502.
[8]  Ferreira M C,Andrade D S,Chueire L M O.Tillage method and crop rotation effects on the population sizes and diversity of Bradyrhizobia nodulating soybean[J].Soil Biology and Biochemistry,2000,32:627-637.
[9]  Hungria M,Vargas M A T.Environmental factors affecting N2?fixation in grain legumes in the tropics,with an emphasis on Brazil[J].Field Crops Research,2000,65:151-164.
[10]  Wang H,Man C X,Wang E T,et al.Diversity of rhizobia and interactions among the host legumes and rhizobial genotypes in an agricultural-forestry ecosystem[J].Plant Soil,2009,314:169-182.
[11]  Han L L,Wang E T,Han T X.Unique community structure and biogeography of soybean rhizobia in the saline-alkaline soils of Xinjiang,China[J].Plant Soil,2009,324:291-305.
[12]  Ferguson G P,Indrasumunar A,Hayashi S,et al.Molecular analysis of legume nodule development and autoregulation[J].Journal of Integrative Plant Biology,2010,52:61-76.
[13]  Magori S,Kawaguchi M.Long-distance control of nodulation:Molecules and models[J].Molecular Cells,2009,27:129-134.
[14]  Barbulova A,Rogato A,D′Apuzzo E,et al.Differential effects of combined N sources on early steps of the nod factor-dependent transduction pathway in Lotus japonicus[J].Molecular Plant-Microbe Interactions,2007,20(8):994-1003.
[15]  Patriarca E J,Tate R,Iaccarino M.Key role of bacterial NH+4?metabolism in Rhizobium-plant symbiosis[J].Microbiology and Molecular Biology Reviews,2002,66:203-222.
[16]  张兴义,王树奎,隋跃宇.东北农田黑土碱解氮现状评价[J].农业系统科学与综合研究,2005,21(4):305-309.(Zhang X Y,Wang S K,Sui Y Y.Evolution of alkali dispelled nitrogen of black soil in Northeast China[J].System Sciences and Comprehensive Studies in Agriculture,2005,21(4):305-309.)
[17]  郭海龙,马春梅,董守坤,等.春大豆生长中对不同氮源的吸收利用[J].核农学报,2008,22(3):338-342.(Guo H L,Ma C M,Dong S K,et al.Absorption and utilization of different nitrogen sources during the growth of soybean plant[J].Journal of Nuclear Sciences,2080,22(3):338-342.)

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133