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双氢青蒿素抑制胶质瘤细胞生长机制

DOI: 10.11847/zgggws2015-31-11-09, PP. 1396-1398

Keywords: 双氢青蒿素(DHA),胶质瘤细胞,cyclinD1,Bax/Bcl-2,caspase-3

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Abstract:

?目的探讨双氢青蒿素对胶质瘤细胞C6生长影响及可能机制。方法培养大鼠脑胶质瘤C6细胞,噻唑蓝法检测双氢青蒿素对C6细胞生长抑制率,annexinV/PI检测C6细胞凋亡率;ELISA法检测细胞培养液上清中cyclinD1、Bax和casepase-3蛋白表达变化;Westernblot法检测Bax和Bcl-2蛋白表达量。结果双氢青蒿素可明显抑制C6细胞生长,以作用48h最为明显,0.05、0.50、5.00和50.00μmol/L双氢青蒿素组细胞生长抑制率分别为(83.96±1.08)%、(74.04±3.54)%、(68.70±9.20)%和(57.25±7.01)%,与对照组(100.00±1.07)%比较,差异有统计学意义(P<0.01);50.00μmol/L双氢青蒿素组细胞凋亡率[(25.98±3.95)%]明显高于对照组[(2.32±0.78)%],差异有统计学意义(P<0.01);与对照组比较,各剂量双氢青蒿素组C6细胞培养液上清中caspase-3分泌增加,50.00μmol/L双氢青蒿素组细胞Bax蛋白表达增加,Bcl-2蛋白表达减少,Bax/Bcl-2比值增加。结论双氢青蒿素通过下调cyclinD1蛋白,上调Bax/Bcl-2比值使caspase-3蛋白活化,从而抑制胶质瘤细胞生长。

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