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重组人乳铁蛋白对小鼠沙门菌感染抑制效果

DOI: 10.11847/zgggws2015-31-09-17, PP. 1166-1169

Keywords: 重组人乳铁蛋白,沙门菌,感染,小鼠

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Abstract:

?目的研究转基因水稻来源的重组人乳铁蛋白(rhLF)在小鼠体内抑制沙门菌感染的效果。方法选择Balb/c小鼠建立沙门菌感染模型;将Balb/c小鼠按体重随机分为2组,即沙门菌对照组和rhLF干预组,每组12只;染菌前2h,rhLF干预组小鼠经口灌胃20mg/mLrhLF0.3mL,对照组小鼠经口灌胃0.3mLPBS;之后每天灌胃相同量的rhLF(6mg/d)或PBS;每天观察小鼠一般情况;7d后,心脏穿刺取小鼠血液观察菌血症,取肝脏和脾脏组织进行细菌计数分析,取肝脏、脾脏、肾脏和小肠进行病理观察。结果各组小鼠体重变化、生存情况在组间无明显差异(P>0.05);2组小鼠的肝脏重和肝体比值、脾脏重和脾体比值相似(P>0.05);rhLF干预组小鼠的血液、肝脏和脾脏的染菌率和染菌量与对照组相比,呈现降低趋势,但差异无统计学意义(P>0.05);病理分析显示rhLF干预组小鼠的炎症程度有减轻,但差异无统计学意义(P>0.05)。结论6mg/d的rhLF不能明显减轻小鼠沙门菌感染的炎症程度。

References

[1]  袁安文,潘荞,邓洁汝,等.副猪嗜血杆菌培养基筛选与小鼠半数致死量测定[J].上海畜牧兽医通讯,2011(2):41-42.
[2]  Murray RA,Lee CA.Invasion genes are not required for Salmonella enterica serovar typhimurium to breach the intestinal epithelium:evidence that salmonella pathogenicity island 1 has alternative functions during infection[J].Infect Immun,2000,68:5050-5055.
[3]  Mosquito S,Ochoa TJ,Cok J,et al.Effect of bovine lactoferrin in Salmonella ser.Typhimurium infection in mice[J].Biometals,2010,23:515-521.
[4]  Drago-Serrano ME,Rivera-Aguilar V,Reséndiz-Albor AA,et al.Lactoferrin increases both resistance to Salmonella Typhimurium infection and the production of antibodies in mice[J].Immunology Letters,2010,134:35-46.
[5]  Haraga A,Ohlson MB,Miller SI.Salmonellae interplay with host cells[J].Nat Rev Microbiol,2008,6(1):53-66.
[6]  Nilsson AI,Kugelberg E,Berg OG,et al.Experimental adaptation of Salmonella typhimurium to mice[J].Genetics,2004,168(3):1119-1130.
[7]  Zagulski T,Lipinski P,Zagulska A,et al.Antibacterial system generated by lactoferrin in mice in vivo is primarily a killing system[J].Int J Exp Pathol,1998,79:117-123.
[8]  Artym J,Zimecki M,Kruzel ML.Enhanced clearance of Escherichia coli and Staphylococcus aureus in mice treated with cyclophosphamide and lactoferrin[J].Int Immunopharmacol,2004,4:1149-1157.
[9]  Bhimani RS,Vendrov Y,Furmanski P.Influence of lactoferrin feeding and injection against systemic staphylococcal infections in mice[J].Journal of Applied Microbiology,1999,86:135-144.
[10]  Lee HY,Park JH,Seok SH,et al.Potential antimicrobial effects of human lacto-ferrin against oral infection with Listeria monocytogenes in mice[J].J Med Microb,2005,54:1049-1054.
[11]  Dial EJ,Romero JJ,Headon DR,et al.Recombinant human lacto-ferrin is effective in the treatment of Helicobacter felis-infected mice[J].J Pharm Pharmacol,2000,52:1541-1546.
[12]  Edde L,Hipolito RB,Hwang FF,et al.Lactoferin protects neonatal rats from gut related systemic infection[J].Am J Physiol Gastrointest Liver Physiol,2001,281:G1140-1150.
[13]  Appelmelk BJ,An YQ,Geerts M,et al.Lactoferrin is a lipid A-binding protein[J].Infect Immun,1994,62(6):2628-2632.
[14]  Brandenburg K,Jurgens G,Muller M,et al.Biophysical characterization of lipopolysaccharide and lipid A inactivation by lactoferrin[J].Biol Chem,2001,382(8):1215-1225.
[15]  Ellison RT,Giehl TJ,Laforce FM.Damage of the outer membrane of enteric gram-negative bacteria by lactoferrin and transferrin[J].Infect Immun,1988,56(11):2774-2781.
[16]  Garcia-montoya IA,Cendon TS,Arevalo-gallegos S,et al.Lactoferrin a multiple bioactive protein:an overview[J].Biochim Biophys Acta,2012,1820(3):226-236.
[17]  吴景欢,杨丽琛,刘改革,等.转基因水稻中重组人乳铁蛋白的分离纯化及其抑菌活性研究[J].卫生研究,2013,42(3):399-404.

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