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外源脯氨酸对盐胁迫下甜瓜幼苗根系抗坏血酸-谷胱甘肽循环的影响

DOI: 10.11913/PSJ.2095-0837.2014.50502, PP. 502-508

Keywords: 甜瓜(CucumismeloL.),脯氨酸,盐胁迫,抗坏血酸-谷胱甘肽循环(ASA-GSH)

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Abstract:

?以2个不同耐盐强度的甜瓜品种‘玉皇’(耐盐性强)和‘雪美’(耐盐性弱)为材料,采用营养液栽培方法,研究外源脯氨酸对盐胁迫下甜瓜幼苗根系抗坏血酸-谷胱甘肽循环的影响。结果显示:(1)盐胁迫下,2个甜瓜品种根系内的还原型抗坏血酸(ASA)、还原型谷胱甘肽(GSH)含量降低,氧化型谷胱甘肽(GSSG)含量升高,且‘雪美’变化幅度大于‘玉皇’;(2)盐胁迫下,施用外源脯氨酸提高了2个甜瓜品种根系中ASA和GSH的含量,降低了GSSG含量,同时也提高了GSH/GSSG的比值,且对‘雪美’的作用大于‘玉皇’;(3)盐胁迫处理3d时,2个甜瓜品种根系的抗坏血酸过氧化物酶(APX)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)活性均下降,且‘雪美’下降的幅度较大;随着胁迫时间的延长(5d时),‘玉皇’幼苗根系内APX、DHAR、GR活性有所上升,‘雪美’根系中这3种酶活性则进一步降低;(4)盐胁迫下,施用外源脯氨酸提高了2个甜瓜品种根系内的APX、DHAR和GR的酶活性,且对‘雪美’的作用大于‘玉皇’。本研究结果表明,外源脯氨酸可以通过增加非酶促抗氧化物质ASA、GSH的含量和抗氧化酶活性,提高抗坏血酸-谷胱甘肽循环清除活性氧的能力,从而缓解盐胁迫对甜瓜植株的伤害。

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