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OALib Journal期刊
ISSN: 2333-9721
费用：99美元
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界面活化的溶胶凝胶包埋Candidarugosa脂肪酶催化合成维生素E琥珀酸酯
DOI: 10.1016/S1872-2067(12)60628-7, PP. 1608-1616
Keywords: 溶胶凝胶,界面活化,Candidarugosa脂肪酶,维生素E琥珀酸酯
Abstract:
？采用界面活化的溶胶凝胶包埋Candidarugosa脂肪酶(CRL)催化合成了维生素E琥珀酸酯.考察了影响溶胶凝胶包埋固定化CRL的因素,获得的最佳固定化条件为:丙基三甲氧基硅烷/正硅酸四乙酯摩尔比为1/1,水与硅烷前体摩尔比为15,酶的添加量为0.5mg/ml,PEG400的添加量为12μl/ml溶胶.溶胶凝胶包埋的CRL在50℃,18h后其活性仍然保持了70.58%,是游离酶的2.6倍,且稳定性得到了明显的改善.基于CRL的界面特性,采用五种表面活性剂对其进行界面活化.结果表明,采用橄榄油活化的溶胶凝胶包埋的CRL合成维生素E琥珀酸酯的酯化活力最高,相比原酶和未界面活化的溶胶凝胶包埋酶分别提高了6.7和1.43倍.
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