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OALib Journal期刊
ISSN: 2333-9721
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哈茨木霉CGMCC2979生物转化栀子中的京尼平苷制备京尼平
DOI: 10.1016/S1872-2067(14)60134-0, PP. 1534-1546
Keywords: 京尼平苷,京尼平,哈茨木霉,生物转化,京尼平苷β-葡萄糖苷酶,纯化
Abstract:
？采用微生物直接转化药材的方法，将栀子中的京尼平苷转化为京尼平，无需糖苷酶和京尼平苷的制备.在培养温度为30℃，pH6.1以及栀子载量为80g/L的条件下，48h京尼平苷的转化率为97.8%.转化后的京尼平通过XAD-16N大孔树脂偶联硅胶层析的方法，制备得到纯度大于95%的京尼平，收率为62.3%.在催化、转化机制研究中，从哈茨木霉CGMCC2979的发酵液中分离得到了分子量为74.4kDa的京尼平苷β-葡萄糖苷酶，该酶最优催化条件为50℃和pH4.0-5.0.Km和Vmax分别为3.6mmol/L和775μmol/h/mg蛋白.本文提供了一种简便、高效制备京尼平的新方法.
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