|
OALib Journal期刊
ISSN: 2333-9721
费用：99美元
|
|
|
|
紫外分光光度法表征LipozymeTLIM脂肪酶转酯化活性
DOI: 10.1016/S1872-2067(14)60053-X, PP. 553-559
Keywords: 脂肪酶,LipozymeTLIM,转酯化活性,紫外分光光度法,棕榈酸对硝基苯酯,有机溶剂
Abstract:
？建立了一种新的有机相脂肪酶转酯化活性测定方法.以正己烷为溶剂，脂肪酶催化棕榈酸对硝基苯酯和正丁醇的转酯化反应为模型反应，通过测定反应液中310nm下吸光值的变化计算反应转化率.以气相色谱法对新建的紫外分光光度法进行验证，分别采用这两种方法测定了七种商品化脂肪酶的转酯化活性，两种方法所得实验结果基本一致.利用紫外分光光度检测法考察了LipozymeTLIM脂肪酶催化转酯化的时间进程及合成活性与酶量的关系，并对LipozymeTLIM催化转酯化的性质（最适溶剂、酰基受体特异性、醇耐受性、最优反应温度和热力学稳定性）进行了表征.
| [1] | Angkawidjaja C, Matsumura H, Koga Y, Takano K, Kanaya S. J Mol Biol, 2010, 400: 82
|
| [2] | Céliz G, Martearena M R, Scaroni E, Daz M. Biochem Eng J, 2012, 69: 69
|
| [3] | Hama S, Tamalampudi S, Yoshida A, Tamadani N, Kuratani N, Noda H, Fukuda H, Kondo A. Biochem Eng J, 2011, 55: 66
|
| [4] | Yang T K, Fruekilde M B, Xu X B. J Am Oil Chem Soc, 2003, 80: 881
|
| [5] | Christensen M W, Andersen L, Husum T L, Kirk O. Eur J Lipid Sci Technol, 2003, 105: 318
|
| [6] | Lomolino G, Di Pierro G, Lante A. Food Technol Biotechnol, 2012, 50: 479
|
| [7] | Palacios D, Busto M D, Ortega N. Lwt-Food Sci Technol, 2014, 55: 536
|
| [8] | Torres M, Dolcet M M, Sala N, Canela R. J Agric Food Chem, 2003, 51: 3328
|
| [9] | Schmidt M, Bornscheuer U T. Biomol Eng J, 2005, 22: 51
|
| [10] | Wang B, Tang X L, Ren G F, Liu J, Yu H W. Biochem Eng J, 2009, 46: 345
|
| [11] | Konarzycka-Bessler M, Bornscheuer U T. Angew Chem Int Ed, 2003, 42: 1418
|
| [12] | Teng Y, Xu Y. Anal Biochem, 2007, 363: 297
|
| [13] | Goujard L, Villeneuve P, Barea B, Lecomte J, Pina M, Claude S, Le Petit J, Ferré E. Anal Biochem, 2009, 385: 161
|
| [14] | Shintre M S, Ghadge R S, Sawant S B. J Chem Technol Biotechnol, 2002, 77: 1114
|
| [15] | Wang H, Zong M H, Wu H, Lou W Y. J Biotechnol, 2007, 129: 689
|
| [16] | Barzegar A, Moosavi-Movahedi A A, Pedersen J Z, Miroliaei M. Enzyme Microb Technol, 2009, 45: 73
|
| [17] | Kumar A, Gross R A. Biomacromolecules, 2000, 1: 133
|
| [18] | Hasan F, Shah A A, Hameed A. Biotechnol Adv, 2009, 27: 782
|
| [19] | Gupta N, Rathi P, Gupta R. Anal Biochem, 2002, 311: 98
|
| [20] | Hriscu M, Chis L, Tosa M, Irimie F D. Eur J Lipid Sci Technol, 2013, 115: 571
|
| [21] | Soumanou M M, Bornscheuer U T. Enzyme Microb Technol, 2003, 33: 97
|
| [22] | Kim I H, Kim H, Lee K T, Chung S H, Ko S N. J Am Oil Chem Soc, 2002, 79: 363
|
| [23] | Romero M D, Calvo L, Alba C, Daneshfar A, Ghaziaskar H S. Enzyme Microb Technol, 2005, 37: 42
|
| [24] | Soumanou M M, Bornscheuer U T. Eur J Lipid Sci Technol, 2003, 105: 656
|
| [25] | Varma M N, Madras G. Appl Biochem Biotechnol, 2010, 160: 2342
|
| [26] | Yadav G D, Pawar S V. Bioresour Technol, 2012, 109: 1
|
| [27] | Yadav G D, Lathi P S. J Mol Catal B, 2005, 32: 107
|
| [28] | Yadav G D, Sivakumar P. Biochem Eng J, 2004, 19: 101
|
| [29] | Talukder M M R, Das P, Fang T S, Wu J C. Biochem Eng J, 2011, 55: 119
|
| [30] | Zhang H, Pedersen L S, Kristensen D, Adler-Nissen J, Holm H C. J Am Oil Chem Soc, 2004, 81: 653
|
| [31] | Khor G K, Sim J H, Kamaruddin A H, Uzir M H. Bioresour Technol, 2010, 101: 6558
|
| [32] | Yadav G D, Thorat P A. J Mol Catal B, 2012, 83: 16
|
| [33] | Zhu S S, Li M, Yu X W, Xu Y. Appl Biochem Biotechnol, 2013, 170: 436
|
| [34] | Ignatjev I, Valincius G, Svedaite I, Gaidamauskas E, Kazemekaite M, Razumas V, Svendsen A. Anal Biochem, 2005, 344: 275
|
| [35] | Jaeger K E, Eggert T. Curr Opin Biotechnol, 2002, 13: 390
|
| [36] | Houde A, Kademi A, Leblanc D. Appl Biochem Biotechnol, 2004, 118: 155
|
| [37] | Yadav G D, Lathi P S. J Mol Catal B, 2004, 27: 113
|
Full-Text
|
|
Contact Us
service@oalib.com QQ:3279437679 
WhatsApp +8615387084133
|
|
