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草业学报  2009 

镉胁迫下蚕豆幼苗抗氧化能力对外源NO和H2O2的响应

, PP. 186-191

Keywords: 一氧化氮,双氧水,蚕豆幼苗,镉胁迫,抗氧化能力

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Abstract:

以蚕豆品种临蚕2号为试验材料,采用土培法,研究了在镉胁迫期间蚕豆幼苗抗氧化酶(SOD、CAT和POD)活性、脯氨酸、丙二醛和活性氧含量的动态变化过程,以探讨外源NO和H2O2对镉毒害下蚕豆幼苗的保护效应。结果表明,0.1mmol/LSNP(外源NO供体)和0.01mmol/LH2O2单独处理均可缓解0.1mmol/L镉对蚕豆幼苗的氧化损伤,而二者互作处理存在正协同效应,主要表现在增强了蚕豆幼苗的保护酶活性,其中SOD在镉胁迫72h后活性达到峰值,比对照提高57.89%,CAT和POD均在镉胁迫48h后活性达到最大值,分别比对照提高69.60%和101.18%;镉胁迫120h时,与单镉胁迫处理相比,互作处理的脯氨酸含量提高了134.88%,而MDA含量、内源H2O2含量和体内O2·-的产生速率下降,有效地增强了蚕豆幼苗在镉胁迫期间的抗氧化能力。

References

[1]  王云, 陈尧, 钱亚如, 等. 镉胁迫对不同品种小麦幼苗生长和生理特性的影响[J]. 生态学杂志, 2008, 27(5): 767-770.
[2]  Sondès R, Abdelilah C, Ezzeddine E F. Differential sensitivity to cadmium in germinating seeds of three cultivars of faba bean (Vicia faba L.)[J]. Acta Physiologiae Plantarum, 2008, 30(4): 451-456.
[3]  Vishnu P G, Ouki S K, Tony H. Remediation of copper and cadmium in contaminated soils using compost with inorganic amendments[J]. Water, Air ﹠Soil Pollut, 2009, 196(1-4): 355-368.
[4]  李清泉, 王成, 王芳, 等. 蚕豆的开发利用及高产栽培技术[J]. 杂粮作物, 2008, 28(2): 121-122.
[5]  Magorzata K, Edward A G. The role of nitric oxide in plant growth regulation and responses to abiotic stresses[J]. Acta Physiologiae Plantarum, 2004, 26(4): 459-472.
[6]  Beligni M V, Lamattina L. Is nitric oxide toxic or protective[J]. Trends Plant Science, 1999, 4(8): 229-300.
[7]  Beligni M V, Lamattina L. Nitric oxide protects against cellular damage produced by methylviologen herbicides in potato plants[J]. Nitric Oxide, 1999, 3(3): 199-208.
[8]  阮海华, 沈文飚, 叶茂炳, 等. 外源一氧化氮对小麦叶片盐胁迫的保护效应[J]. 科学通报, 2001, 46(23): 1993-1997.
[9]  Zhao L Q, Zhang F, Zhang L X. Nitric oxide functions as a signal in salt resistance in the calluses two ceotypes of reed[J]. Plant Physiology, 2004, 134: 849-857.
[10]  Kopyra M, Gwozdz E A. Nitric oxide stimulates seed germination and counteracts the inhibitory effect of heavy metals and salinity on root growth of Lupinus luteus[J]. Plant Physiology Biochemistry, 2003, 41: 1011-1017.
[11]  Hsu Y T, Kao K H. Cadmium toxicity is reduced by nitric oxide in rice leaves[J]. Plant Growth Regulation, 2004, 42: 227-238.
[12]  段凯旋, 杨洪强, 冉昆, 等. 一氧化氮对铜、镉胁迫下平邑甜茶幼苗活性氧代谢的影响[J]. 中国农学通报, 2007, 23(10): 104-109.
[13]  苏桐, 魏小红, 丁学智, 等. 外源NO与蔗糖对盐胁迫下番茄幼苗氧化损伤的保护效应[J]. 生态学报, 2008, 28(4): 1558-1564.
[14]  Xu M J, Dong J F, Zhang X B.Signal interaction between nitric oxide and hydrogen peroxide in heat shock-induced hypericin production of Hypericum perforatum suspension cells[J]. Science China Series C-Life Science, 2008, 51(8): 676-686.
[15]  Neil S J, Desikan R, Clarke A, et al. Hydrogen peroxide and nitric oxide as signaling molecules in plants[J]. Journal of Experimental Botany, 2002, 53: 1237-1247.
[16]  李儒佳, 李雪梅. 水杨酸、脱落酸和过氧化氢对镉胁迫小麦幼苗光合及抗氧化酶活性的影响[J]. 生态学杂志, 2007, 26(12): 2096-2099.
[17]  刘新, 张蜀秋, 娄成后. 气孔运动调控中过氧化氢和一氧化氮信号途径的交叉作用[J]. 自然科学进展, 2003, 13(4): 355-359.
[18]  张志良, 翟伟菁. 植物生理实验指导[M]. 北京: 高等教育出版社, 2004.123-124.
[19]  Dhindsa R S, Plumb-Dhindsa P, Thorpe T A. Leaf senescence correlated with increased levels of membrane permeability and lipid peroxidation and decreased levels dismutase and catalase[J]. Journal of Experimental Botany, 1982, 32: 91-101.
[20]  邹琦. 植物生理学实验指导[M]. 北京: 中国农业出版社, 2000. 163-165.
[21]  赵福庚, 刘友良. 大麦幼苗多胺合成比脯氨酸合成对盐胁迫更敏感[J]. 植物生理学报, 2000, 26(4): 243-349.
[22]  邹琦. 植物生理学实验指导[M]. 北京: 中国农业出版社, 2000. 166-170.
[23]  王爱国, 罗广华. 植物的超氧物自由基与羟胺反应的定量关系[J]. 植物生理学通讯, 1990, 6: 55-57.
[24]  王俊红, 魏小红, 龙瑞军, 等. 外源一氧化氮对渗透胁迫下小麦幼苗叶片膜脂过氧化的影响[J]. 甘肃农业大学学报, 2008, 43(1): 77-81.
[25]  王罗霞, 赵志光, 王锁民. 一氧化氮对水分胁迫下小麦叶片活性氧代谢及膜脂过氧化的影响[J]. 草业学报, 2006, 15(4): 104-108.
[26]  周瑞莲, 王刚. 水分胁迫下豌豆保护酶活力变化及脯氨酸积累在其抗旱中的作用[J]. 草业学报, 1997, 6(4): 39-43.
[27]  苏桐, 龙瑞军, 魏小红, 等. 外源NO对NaCl胁迫下燕麦幼苗氧化损伤的保护作用[J]. 草业学报, 2008, 17(5): 48-53. 浏览
[28]  Singh N K, Nelson D E, Kuhn D M, et al. Molecular coning of osmotin and regulation of expression by ABA and adaption to low water potentials[J]. Plant Physiology, 1989, 90: 1096-1101.
[29]  马向丽, 魏小红, 龙瑞军, 等. 外源一氧化氮提高一年生黑麦草抗冷机制[J]. 生态学报, 2005, 25(6): 1269-1274.
[30]  张永峰, 殷波. 混合盐碱胁迫对苗期紫花苜蓿抗氧化酶活性及丙二醛含量的影响[J]. 草业学报, 2009, 18(1): 46-50. 浏览
[31]  张庆峰, 徐胜, 李建龙. 高温胁迫下高羊茅生理生化特性研究[J]. 草业科学, 2006, 23(4): 26-28.
[32]  Inze D, Van M M. Oxidative stress in plants[J]. Current Opinion Biotechnology, 1995, 6: 153-158.
[33]  刘晓静, 柳小妮. 多效唑和烯效唑对草地早熟禾一些生化指标及其抗性的影响[J]. 草业学报, 2006, 15(2): 48-53.
[34]  Valentina M. Activities of SOD and the ascorbate glutathion cycle enzymes in subcellular compartments in leaves and roots of the cultivated tomato and its wild salt tolerant relative Lycopersicon pennellii[J]. Plant Physiology, 2000, 33: 65-77.
[35]  王友保, 张莉, 刘惠, 等. 铜对狗牙根生长及活性氧清除系统的影响[J]. 草业学报, 2007, 16(1): 52-57.
[36]  Asada K, Takahashi M. Production and Scavenging of Active Oxygen in Photosynthesis Amsterdam[M]. Elsevier Science Publishers, 1987.227-287.
[37]  张文利, 沈文飚, 叶茂炳, 等. 小麦叶片顺乌头酸酶对NO和H2O2的敏感性[J]. 植物生理与分子生物学学报, 2002, 28(2): 99-104.
[38]  李梦梅, 龙明化. 植物抗镉胁迫的研究综述[J]. 广西农业科学, 2005, 36(4): 319-322

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