全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
草业学报  2009 

甘肃中部地区禾谷镰孢的变异研究

, PP. 118-124

Keywords: 禾谷镰孢,子囊壳,真菌毒素,Fg16,Tri13,PCR

Full-Text   Cite this paper   Add to My Lib

Abstract:

为掌握禾谷镰孢在甘肃中部地区的分布及变异情况,从根部表现有坏死和叶鞘发褐的小麦幼苗的不同部位、小麦地土壤及玉米籽粒、玉米茎秆上分离禾谷镰孢,并以形态学为基础,参照Nelson分类系统进行鉴定。结果表明,在分离到的43个镰刀菌菌株中,有14个菌株经鉴定为禾谷镰孢,均从玉米茎秆上分离到,小麦根部、小麦叶鞘、小麦地土壤、玉米籽粒中分离到的镰刀菌中未见禾谷镰孢。将禾谷镰孢在特定条件下培养后,发现14个禾谷镰孢菌株产生子囊壳的数量不同,为2~90个。在以Fg16为引物的PCR反应中,随机选取的11个禾谷镰孢菌株都产生0.41kb的PCR产物,而6个对照菌株都产生0.50kb的片段,证明引物Fg16可以区分禾谷镰孢菌株群体的遗传多态性。以Tri13为引物的PCR反应显示,11个禾谷镰孢菌株以及3个中国对照菌株都产生脱氧雪腐镰刀菌烯醇(DON)毒素,而3个澳大利亚对照菌株产生雪腐镰刀菌烯醇(NIV)毒素。

References

[1]  Rubella S G, Kistler H C. Heading for disaster: Fusarium graminearum on cereal crops[J]. Molecular Plant Pathology, 2004, 5(6): 515-525.
[2]  Sutton J C. Epidemiology of wheat head blight and maize ear rot caused by Fusarium graminearum[J]. Canadian Journal of Plant Pathology, 1982, 4: 195-209.
[3]  Parry D W, Jenkinson P, Mclead L. Fusarium ear blight (scab) in small grain cereals-a review[J]. Plant Pathology, 1995, 44: 207-238.
[4]  Blaney B J, Dodman R L. Production of zearalenone, deoxynivalenol, nivalenol, and acetylated derivatives by Australian isolates of Fusarium graminearum and F. pseudograminearum in relation to source and culturing conditions[J]. Australian Journal of Agricultural Research, 2002, 53: 1317-1326.
[5]  O’Donnell K, Kistler H C, Tacke B K, et al. Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab[A]. Proceedings of the National Academy of Sciences of the United States of America[C]. Washington:University of Washington Press, 2000.
[6]  Williams K C, Blaney B J. Effect of the mycotoxins, nivalenol and zearalenone, in maize naturally infected with Fusarium graminearum on the performance of growing and pregnant pigs[J]. Australian Journal of Agricultural Research, 1994, 45: 1265-1279.
[7]  Dusabenyagasani M, Dostaler D, Hamelin R C. Genetic diversity among Fusarium graminearum strains from Ontario and Quebec[J]. Canadian Journal of Plant Pathology, 1999, 21: 308-314.
[8]  Jennings P, Coates M E, Walsh K, et al. Determinaiton of deoxynivalenol and nivalenol-producing chemotypes of Fusarium graminearum isolated from wheat crops in England and Wales[J]. Plant Pathology, 2004, 53: 643-652.
[9]  Gale L R, Chen L F, Hernick C A, et al. Population analysis of Fusarium graminearum from wheat fields in eastern China[J]. Phytopathology, 2002, 92: 1315-1322.
[10]  胡光荣, 赵纯森, 廖玉才. 我国小麦禾谷镰刀菌(Fusarium graminearum)营养体亲合性与致病性研究[J].植物病理学报,2003,33(4):379-380.
[11]  赵纯森,马星霞,武爱波,等.禾谷镰刀菌培养性状与致病力的相关性分析[J]. 华中农业大学学报,2005, 24(3):254-257.
[12]  Wang Y Z. Epidemiology and management of wheat scab in China[A]. Fusarium Head Scab: Global Status and Future Prospects[M]. Mexico: Mexico D F, 1997.
[13]  Tio M, Burgess L W, Nelson P E, et al. Techniques for the isolation, culture and preservation of the Fusaria[J]. Australian Plant Pathology Society Newsletter, 1977, 6: 11-13.
[14]  Fisher N L, Burgess L W, Tousson T A, et al. Carnation leaves as a substrate for preserving cultures of Fusarium species[J]. Phytopathology, 1982, 72: 151-153.
[15]  Burgess L W, Sumerell B A, Bullock S, et al. Laboratory Manual for Fusarium Research(3rd ed)[M]. Sydney: Sydney University Press, 1994.
[16]  Knight T. Genetic and phenotypic variation between two geographically isolated populations of Fusarium graminearum in Australia(0228578)[R]. Sydney: Sydney University Press, 2005.
[17]  Smith W J, Brett A S, Gunn L, et al. Molecular Techniques for the Study of Soilborn Fungal Plant Panthology[M]. Sydney: Sydney University Press, 2003.
[18]  Chandler E A, Duncan R S, Thomsett M A, et al. Development of PCR assays to Tri7 and Tri13 and characterisation of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis[J]. Physiological and Molecular Plant Pathology, 2003, 62: 355-367.
[19]  Walker S L, Leath S, Hagler W M, et al. Variation among isolates of Fusarium graminearum associated with Fusarium head blight in North Carolina[J]. Plant Disease, 2001, 85: 404-410.
[20]  Cook R J. Fusarium: Diseases, Biology, and Taxonomy[M]. University Park, PA, Pennsylvania State University Press, 1981.
[21]  Carter J P, Rezanoor H N, Desjardins A E, et al. Variation in Fusarium graminearum isolates from Nepal associated with their host of origin[J]. Plant Pathology, 2000, 49: 452-460.
[22]  Carter J P, Rezanoor H N, Holden D, et al. Variation in pathogenicity associated with the genetic diversity of Fusarium graminearum[J]. European Journal of Plant Pathology, 2002, 108(6): 573-583.
[23]  Desjardins A E, Plattner R D. Diverse traits for pathogen fitness in Gibberella zeae[J]. Canadian Journal of Plant Pathology, 2003, 25: 21-27.
[24]  Zeller K A, Bowden R L, Leslie J F. Population differentiation and recombination in wheat scab populations of Gibberella zeae from the United States[J]. Molecular Ecology, 2004, 13: 563-571.
[25]  曲涛,南志标.作物和牧草对干旱胁迫的响应及机理研究进展[J].草业学报,2008,17(2):126-135.
[26]  王生荣,郑华平,何冬云,等. 天祝高寒草原草地植物黑粉病调查研究[J].草业学报,2008,17(1):48-51.
[27]  张礼军,张恩和,郭丽琢,等. 水肥耦合对小麦/玉米系统根系分布及吸收活力的调控[J].草业学报,2005,14(2):102-108.
[28]  陈本建. 甘肃省豆科牧草资源及植物区系分析[J].草业科学,2008,25(4):42-45.
[29]  刘生瑞,常生华,侯扶江. 甘肃省退耕还林还草区划刍议[J].草业科学,2008,25(1):5-9

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133