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草业学报  2009 

柱花草种质遗传多样性的ISSR分析

, PP. 57-64

Keywords: 柱花草,种质,遗传多样性,ISSR

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Abstract:

应用ISSR标记,对48份柱花草种质的遗传多样性进行研究。从68个ISSR引物中筛选出14个多态性明显、反应稳定的引物,共扩增出156条谱带,平均每个引物能扩增出11.1条带,多态性条带比率达99.36%。材料间遗传相似系数为0.445~0.975,POPGENE结果分析表明,平均Shannon信息指数(I)为0.3553,平均Nei’s基因多样性(H)为0.2279,每位点平均有效等位基因数(NE)为1.3887。利用聚类分析和主成分分析表明,48份供试材料可聚为5类:有钩柱花草类、头状柱花草类、圭亚那柱花草类、西卡柱花草类和灌木柱花草类,其中,圭亚那柱花草类种内材料遗传变异较大。

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