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草业学报  2010 

菊苣种质资源遗传多样性的SRAP研究

, PP. 139-147

Keywords: 菊苣,种质资源,SRAP,遗传多样性

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Abstract:

利用SRAP分子标记技术对来自5大地理类群的48份菊苣材料的遗传多样性进行了研究。用筛选的28对引物对48份材料的DNA进行PCR扩增,获得以下结论:1)28个位点共检测到333个等位基因,平均为11.89个,多态性位点率(P)平均为95.09%;多态性信息含量(PIC)范围为0.23(me3+em3)~0.44(me1+em10),平均为0.33;2)材料间遗传相似系数(GS)范围在0.55~0.89,平均GS值为0.69;地理类群间的GS值在0.63~0.96,其中荷兰(P,95.52%)和意大利(P,95.38%)类群遗传多样性丰富,表明供试菊苣种质具有丰富的遗传变异;3)根据研究结果进行聚类分析和主成分分析,可将48份菊苣材料分为五大类,来自相同地理类群的材料基本能聚为一类,呈现出一定的地域性分布规律。

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