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草业学报  2010 

γ射线对沟叶结缕草愈伤组织再生和耐盐性的影响

, PP. 25-30

Keywords: 沟叶结缕草,10Gy,60Coγ射线,再生,NaCl

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Abstract:

以沟叶结缕草匍匐茎诱导的胚性愈伤组织为材料,分别进行0Gy(对照)和10Gy的60Coγ射线辐照处理,比较愈伤组织的再生能力以及再生植株的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性和脯氨酸含量,并对辐射材料进行不同浓度(0%,0.5%,1.0%,1.5%和2.0%)的NaCl筛选。结果表明,10Gy60Coγ射线处理对愈伤组织的再生分化有显著的促进作用,分化形成3个以上小苗的外植体比率提高11.25%,但是愈伤组织再生率下降8.75%;再生植株的CAT活性提高16.02%,脯氨酸含量提高1.20倍;同对照相比,10Gyγ射线处理的愈伤组织获得的再生植株中SOD和POD活性没有显著变化。10Gy60Coγ射线辐射处理能显著提高愈伤组织对NaCl的抗性;外植体的生长速度和存活率随着NaCl浓度的提高而下降;愈伤组织经过10Gy60Coγ射线处理后,在含1.0%NaCl的再生培养基上获得最多的耐盐植株(成苗率为9.60%),这些植株转移到含1.0%NaCl的生根培养基上后保持旺盛生长。

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