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草业学报  2010 

文心兰组织培养及转基因研究进展

, PP. 220-229

Keywords: 文心兰,组织培养,体细胞胚胎发生,转基因,研究进展

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Abstract:

综述了文心兰组织培养及转基因研究的主要进展。外植体、培养基及其添加物、植物生长调节剂和培养方式对文心兰类原球茎或丛芽诱导均产生重要影响,大田植株的花梗、花芽、茎尖、根尖等均可用作初代培养的外植体,其中花梗及花芽为最常用的外植体,试管苗的所有组织器官均是有效的外植体;文心兰离体培养以固体培养方式为主,1/2MS、MS是用于文心兰离体培养最常用的基本培养基,培养基中植物生长调节剂种类、浓度及其配比是文心兰离体培养成功与否的关键因素。文心兰类原球茎形成过程是典型的体细胞胚胎发生发育过程,体细胞胚胎发生受植物生长调节剂、基因型、培养条件等影响较大。文心兰转基因研究尚处于初步阶段。

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