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草业学报  2010 

青藏高原东南缘老芒麦自然居群遗传多样性的SRAP和SSR分析

, PP. 122-134

Keywords: 老芒麦,遗传多样性,群体遗传结构,SRAP,SSR

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Abstract:

基于SRAP和SSR分子标记分析了青藏高原东南缘8个老芒麦自然居群遗传变异及群体遗传结构,获得下述结果:1)16对SRAP引物在90个单株中共扩增出384条可统计条带,其中多态性条带334条,占86.98%;16个SSR位点共检测出等位变异221个,平均每个位点13.8个,其中具有多态性的位点数192个,占86.88%。2)2种分子标记检测到老芒麦居群水平的基因多样性(He)分别为0.1092和0.1296,而物种水平的基因多样性达0.2434和0.3732。3)基于2种标记的Nei氏遗传分化指数Gst(0.5525和0.5158)表明老芒麦居群出现了较大程度的遗传分化,居群间的基因流非常有限,分别为0.4050和0.4694,老芒麦的遗传变异主要分布在居群间,居群内变异相对较小,Shannon多样性指数和分子方差变异(AMOVA)分析显示了相似的结果。4)基于聚类分析结果表明各居群间存在较为明显的地理分化,8个居群分化为采集地范围内的南、北和中部3个分支。通过对老芒麦遗传多样性和遗传结构的分析提出了对该物种遗传多样性的保护策略。

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