全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
草业学报  2013 

添加外源纤维水解酶对羊草体外瘤胃发酵酶活性及特性的影响

, PP. 315-321

Keywords: 纤维素酶,木聚糖酶,挥发性脂肪酸,体外试验

Full-Text   Cite this paper   Add to My Lib

Abstract:

试验通过分别添加纤维素酶与木聚糖酶0,10.0与50.0mg水平,0与24h两个预处理时间,每个处理3个重复,体外法评价纤维素酶与木聚糖酶复合处理羊草后与瘤胃液共培养对木聚糖酶与葡聚糖酶活性及发酵特性的影响。结果表明,添加纤维水解酶能提高0与8h培养液中木聚糖酶和内切葡聚糖酶活性以及0h培养液中外切葡聚糖酶活性,且具有添加剂量效应。当培养至24及48h,添加外源酶制剂并不能提高培养液中相关酶活性。外源酶制剂显著增加培养24与48h发酵液中乙酸含量,8,24与48h总挥发性脂肪酸产量(P<0.05)以及48h累积产气量(P<0.05),但对培养期内戊酸与异戊酸含量没有影响(P>0.05)。结果提示添加外源酶制剂能提高早期培养液中木聚糖酶和葡聚糖酶活性、增加VFA产量和改善体外瘤胃发酵特性。

References

[1]  刁其玉.酶制剂在反刍动物日粮中应用研究进展.饲料与畜牧,2010,3:15-17.
[2]  冯仰廉.反刍动物营养学.北京:科学出版社,2004:2-64.
[3]  Hristov A N, McAllister T A, Chen K J. Stability of exogenous polysaccharide-degrading enzymes in the rumen. Animal Feed Science and Technology, 1998, 76: 161-168.
[4]  Yang W Z, Beauchemin K A, Rode L M. Effects of an enzyme feed additive on extent of digestion and milk production of lactating dairy cows. Journal of Dairy Science, 1999, 82(2): 391-403.
[5]  Morgavi D P, Beauchemin K A, Nsereko V L, et al. Synergy between ruminal fibrolytic enzymes and enzymes from Trichoderma longibrachiatum. Journal of Dairy Science, 2000, 83(6): 1310-1321.
[6]  Beauchemin K A, Rode L M, Sewalt V J H. Fibrolytic enzymes increase fiber digestibility and growth rate of steers fed dry forages. Canada Journal of Animal Science, 1995, 5: 641-644.
[7]  Kung L J, Treacher R J, Nauman G A, et al. The effect of treating forages with fibrolytic enzymes on its nutritive value and lactation performance of dairy cows. Journal of Dairy Science, 2000, 83(1): 115-122.
[8]  陈鑫珠,庄益芬,张建国,等.生物添加剂对水葫芦与甜玉米秸秆混合青贮品质影响.草业学报, 2011, 20(6): 195-202. 浏览
[9]  Colombatto D, Mould F L, Bhat M K, et al. In vitro evaluation of fibrolytic enzymes as additives for maize (Zea mays L.) silage II. Effects on rate of acidification, fibre degradation during ensiling and rumen fermentation. Animal Feed Science and Technology, 2004, 111: 129-143.
[10]  Russel J B, Martin S A. Effects of various methane inhibitors on the fermentation of amino acids by mixed rumen microorganisms in vitro. Journal of Animal Science, 1984, 59: 1329-1338.
[11]  Wood T M, Bhat M K. Methods for Measuring Cellulase Activities. Methods in Enzymology. In: Wood W A, Kellogg S T. London, UK: Academic Press Inc., 1988: 87-111.
[12]  Pinos-Rodriguez J M, Gonzalez S S, Mendoza G D, et al. Effect of exogenous fibrolytic enzyme on ruminal fermentation and digestibility of alfalfa and rye-grass hay fed to lambs. Journal of Animal Science, 2002, 80(11): 3016-3020.
[13]  Beauchemin K A, Colombatto D, Morgavi D P, et al. Use of exogenous fibrolytic enzymes to improve feed utilization by ruminants. Journal of Animal Science, 2003, 81(E.suppl.2): 37-47.
[14]  Wang Y, McAllister T A, Rode L M, et al. Effects of an exogenous enzyme preparation on microbial protein synthesis, enzyme activity and attachment to feed in the rumen simulation technique (Rusitec). British Journal of Nutrition, 2001, 85: 325-332.
[15]  Wallace R J, Wallace S J A, McKain N, et al. Influence of supplementary fibrolytic enzymes on the fermentation of corn and grass silages by mixed ruminal micrioorganisms in vitro. Journal of Animal Science, 2001, 79(7): 1905-1916.
[16]  Tang S X, Yayo G O, Tam Z L, et al. Effects of yeast culture and fibrolytic enzyme supplementation on in vitro fermentation characteristic of low-quality cereal straws. Journal of Animal Science, 2008, 86(5): 1164-1172.
[17]  秦为琳.应用气相色谱测定瘤胃液挥发性脂肪方法的研究改进.南京农学院学报,1982, 4:110-116.
[18]  Arriola K G, Kim S C, Staples C R, et al. Effect of fibrolytic enzyme application to low-and- concentrate diets on the performance of lactating dairy cattle. Journal of Dairy Science, 2011, 94(2): 832-841.
[19]  Arriola K G, Adesogan A T, Kim S C, et al. Effect of fibrolytic enzyme application to diets differing in concentrate proportion on the performance of lactating dairy cattle. Journal of Animal Science, 2007, 85(Suppl 1): 304.
[20]  House B P, Holden L, Varga G A. Effects of Bovazyme WPTM on microbil efficiency and metabolism in contunousculture of remen contents. Journal of Animal Science, 2007, 85(Suppl 1): 301.
[21]  Lewis G E, Hunt C W, Sanchez W K, et al. Effect of direct-fed fibrolytic enzymes on the digestive characteristics of a forage-based diet fed to beef steers. Journal of Animal Science, 1996, 74(12): 3020-3028.
[22]  Beauchemin K A, Yang W Z, Rode L M. Effects of grain source and enzymen additive on site and extent of nutrient in dairy cows. Journal of Dairy Science, 1999, 82(2): 378-390.
[23]  Hristov A N, McAllister T A, Cheng K J. Intraruminal supplementation with increasing levels of exogenous polysaccharide- degrading enzymes: Effects on nutrient digestion in cattle fed a barley grain diet. Journal of Animal Science, 2000, 78: 477-487.
[24]  Giraldo L A, Tejido M L, Ranilla M J, et al. Influence if direct-fed fibrolytic enzymes on diet digestibility and ruminal activity in sheep fed a grass hay-based diet. Journal of Animal Science, 2008, 86(7): 1617-1623.
[25]  Colombatto D, Mould F L, Bhat M K, et al. Influence of fibrolytic enzymes on the hydrolysis and fermentation of pure cellulose and xylan mixed ruminal microorganisms in vitro. Journal of Animal Science, 2003, 81(4): 1040-1050.
[26]  Fontes C M, Hall J G A, Hirst B H, et al. The resistance of cellulases and xylanases to proteolytic inactivation. Applied Microbiology and Biotechnology, 1995, 43(1): 52-57.
[27]  Hirstov A, McAllister T A, Cheng K J. Effect of dietary or abomasal supplementation of exogenous polysaccharide-degrading enzymes on rumen fermentation and nutrient digestibility. Journal of Animal Science, 1998, 76(12): 3146-3156.
[28]  Morgavia D P, Newbold C J, Beever D E, et al. Stability and stabilization of potential feed additive enzymes in rumen fluid. Enzyme and Microbial Technology, 2000, 26(2-4): 171-177.
[29]  Morgavi D P, Beauchemin K A, Nsserko V L, et al. Resistance of feed enzymes to proteolytic inactivation by rumen microorganisms and gastrointestinal enzymes. Journal of Animal Science, 2001, 79(6): 1621-1630.
[30]  Eun J S, Beauchemin K A.Effects of a proteolytic feed enzyme on intake, digestion, ruminal fermentation, and milk production. Journal of Dairy Science, 2005, 88(6): 2140-2153.
[31]  Nsereko V L, Morgavi D P, Rode M, et al. Effects of fungal enzyme preparations on hydrplysis and sybsequent degradation of alfalfa hay fiber by mixing rumen microorganisms in viro. Animal Feed Science and Technology, 2000, 88: 153-170.
[32]  Nsereko V L, Beauchemin K A, Morgavi D P, et al. Effect of a fibrolytic enzyme preparation from Trichoderma longibrachiatum on the rumen microbial population of dairy cows. Canadian Journal of Microbiology, 2002, 48(1): 14-20.
[33]  Krzysztof B, Magdalena L R. Effect of adding fibrolytic enzymes to dairy cow rations on digestive activity in the rumen. Annals of Animal Science, 2010, 10(2): 127-137.
[34]  Giraldo L A, Tejido M L, Ranilla M J, et al. Effects of exogenous cellulase supplementation on microbial growth and ruminal fermentation of a high-forage diet in Rusitec fermenters. Journal of Animal Science, 2007, 85: 1962-1970.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133