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草业学报  2014 

镉胁迫对白三叶的富集能力、叶片显微结构及其生理特性的影响

DOI: 10.11686/cyxb20140621, PP. 167-175

Keywords: 镉胁迫,白三叶,重金属富集,显微结构,生理特性

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Abstract:

以白三叶为试验材料,对其进行不同浓度Cd2+(0,100,200,300,400,500μmol/L)胁迫7d,结果表明,随Cd2+浓度增加,白三叶各营养器官Cd积累量随之升高,各器官对Cd的富集能力表现为:根>叶>茎;同时,富集在叶片部分的Cd对白三叶叶片产生了毒害作用,使叶肉细胞的细胞壁着色加深,栅栏组织细胞以及海绵组织细胞的形状、大小及细胞排列的疏密程度发生改变。Cd2+胁迫后,白三叶叶片叶绿素含量显著降低(P<0.05),叶片的MDA含量呈上升趋势,且与Cd2+浓度之间存在剂量效应关系;整个胁迫过程中,SOD,POD和CAT活性则呈先升高后降低的趋势。相关性分析结果表明,Chla和Chlb含量与Cd2+浓度间呈极显著负相关;MDA含量与Cd2+浓度间呈极显著正相关;SOD,POD,CAT之间呈极显著正相关,三者发挥协同作用以抵抗逆境毒害,体现了白三叶对重金属Cd的适应性。

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