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草业学报  2014 

连续混合选择下多花黑麦草杂交群体的SSR多样性变化

DOI: 10.11686/cyxb20140541, PP. 345-351

Keywords: 多花黑麦草,混合选择,SSR标记,杂交育种

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Abstract:

本研究利用SSR分子标记对2个多花黑麦草杂交组合5轮改良群体进行遗传变异分析。20对SSR引物在12个育种群体中共扩增出217个条带,多态性条带比率为92.2%。2个杂交群体的不同选择世代中,分别有72.3%和75.2%的遗传变异存在于群体内,27.7%和24.8%的遗传变异存在于各群体间,群体内的遗传变异大于群体间的遗传变异。从带型分析来看,杂交改良群体中双亲共有的条带比例增多,同时缺失双亲特异性条带的比例减少。随着混合选择世代的增加,多态性条带百分率、Shannon遗传多样性指数及Nei’s基因多态性都呈递减趋势,表明群体内的遗传差异随着选择世代的增加呈下降趋势。研究结果将为多花黑麦草杂交选择育种提供基本信息。

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