全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
草业学报  2014 

根际促生菌筛选及其接种剂对箭筈豌豆生长影响的研究

DOI: 10.11686/cyxb20140528, PP. 241-248

Keywords: 促生菌(PGPR),接种剂,箭筈豌豆,生长,根系

Full-Text   Cite this paper   Add to My Lib

Abstract:

通过测定分离自箭筈豌豆和玉米根际4株细菌的固氮酶活性、溶磷量及分泌生长素能力,将其制成植物根际接种剂,并结合半固体培养试验测定接种剂对箭筈豌豆生长的影响。结果表明,菌株J3-1、J1-15和Y16具备溶磷和分泌生长素能力,J1-15的溶磷能力最强,为548.9mg/L,Y16分泌生长素能力最好,达17.8μg/mL,且菌株Y16具较强固氮能力,J3固氮酶活性为366.51C2H4nmol/(mL·h)。与对照组相比,单一菌株制备的接种剂处理(Y16)可使箭筈豌豆地上生物量、地下生物量分别显著增加104.5%和254.1%(P<0.05),复合接种剂处理F(J3-1+J1-15+Y16+J3)使箭筈豌豆地上和地下生物量分别增加76.1%和192.3%。综合各指标,复合接种剂处理效果明显优于单一接种剂,处理F(J3-1+J1-15+Y16+J3)可使箭筈豌豆株高、根长、根表面积、根体积、根系活力,分别较对照增加29.4%,70.0%,174.0%,194.6%,38.3%。这主要是由于菌种间的互作效应造成的。

References

[1]  Reference:
[2]  Adesemoye A O, Torbert H A, Kloepper J W. Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system[J]. Canadian Journal of Microbiology, 2008, 54(10): 876-886.
[3]  Oliveira C A, Alves V M C, Marriel I E. Phosphate-solubilizing microorganisms isolated from rhizosphere of maize cultivated in an oxisol of the Brazilian Cerrado Biome[J]. Soil Biology and Biochemistry, 2009, 41(9): 1782-1787.
[4]  Chen Z X, Ma S W, Liu L. Studies on phosphorus solubilizing activity of a strain of phosphor bacteria isolated from chestnut type soil in China[J]. Bioresource Technology, 2008, 99(14): 6702-6707.
[5]  Hafeez F Y, Yasmin S, Ariani D, et al. Plant growth-promotingbacteria as biofertilizer[J]. Agronomy for Sustainable Development, 2006, 26: 143-150.
[6]  Suneja P, Dudeja S S, Narula N. Development of multiple coinoculants of different biofertilizers and their interaction with plants[J]. Archives of Agronomy and Soil Science, 2007, 53(2): 221-230.
[7]  Chen Z Z, Zhou S S. 333 / A spring vetch Breeding and Popularization[M]. Lanzhou: Gansu Ethnic Publishing House, 1991.
[8]  Wang D, Ren J Z. Pasture science monographs[M]. Nanjing: Jiangsu Science and Technology Publishing House, 1989.
[9]  Li Q. Alpine pastoral Arrow peas and oats mixed experiment[J]. China grassland and forage, 1984, 1(1): 38-41.
[10]  Zhao Q. Northern low-yielding green manure crop cultivation and use of soil practical[M]. Tianjin: Tianjin Science and Technology Translation and Publishing Corporation, 2010.
[11]  Zeng X C, Wang W M, Luo M N, et al. Effects of different element deficiencies on soybean growth and root morphology[J]. Plant Nutrition and Fertilizer Science, 2010, 16(4): 1032-1036.
[12]  Wang S Q, Han X Z, Yan J, et al. Impact of phosphorus deficiency stress on root morphology, nitrogen concentration and phosphorus accumulation of soybean[J]. Chinese Journal of Soil Science, 2010, 41(3): 644-649.
[13]  Ding H, Zhang Z M, Dai L X, et al. Responses of root morphology of peanut varieties differing in drought tolerance to water-deficient stress[J]. Acta Ecologica Sinica, 2013, 33(17): 5169-5176.
[14]  Liu S, Li T X, Ji L, et al. Phosphorus accumulation and root morphological difference of two ecotypes of Pilea sinofasciata grown in different phosphorus treatments[J]. Acta Prataculturae Sinica, 2013, 22(3): 211-217.
[15]  Hafeez F Y, Malik K A.Manual on Biofertilizer Technology[M]. Pakistan: NIBGE, 2000.
[16]  Malik K A, Bilal R. Survival and Colonization of Inoculated Bacteria in Kallar Grass Rhizosphere and Quantification of N2-Fixation[A]. In: Nitrogen Fixation with Nonlegumes[C]. Skinner F A, Bodderand R M, Fendrik I, (Eds). The Netherlands: Kluwer Academic Publishers, 1989: 301-310.
[17]  Yao T, Zhang D G, Hu Z Z. Associative nitrogen-fixing bacteria in the rhizosphere of Avena sativa in an alpine region Ⅰ Isolation and identification[J]. Acta Prataculturae Sinica, 2004, 13(2): 106-111.
[18]  Yao T. Associative nitrogen-fixing bacteria in the rhizosphere of Avena sativa in an alpine region ⅡPhosphate-solubilizing power and auxin production[J]. Acta Prataculturae Sinica, 2004, 13(3): 85-90.
[19]  Ren D M, Zhang X X, Dong D, et al. Studies on taxonomy and microbiostatic activity of antagonistic strain Kc-t99[J]. Biotechnology Bulletin, 2011, 4(4): 153-157.
[20]  Whiting S N, Neumann P M, Baker J M. Applying a solute transfer model to phytoextraction; Zinc acquisition by Thaspi caerulescens[J]. Plant Soil, 2003, 249: 45-56.
[21]  Hamdali H, Ouhdouch Y. Rock phosphate solubilizing Actinomycetes Screening for plant growth-promoting activities[J]. World Journal of Microbiology and Biotechnology, 2008, 24: 2565-2575.
[22]  Mirz M S, Rasul G, Mehnaz S. Beneficial effects of inoculated nitrogen-fixing bacteria on rice[J]. Biology and Fertility of Soils, 2000, 31: 191-204.
[23]  Oliveira A M, Urquiaga S, Dobereiner J, et al. The effect of inoculating endophytic N2-fixing bacteria on micropropagated sugarcane plants[J]. Plant Soil, 2002, 242: 205-215.
[24]  Hu J C, Xue D L, Ma C X. Research advances in plant growth-promoting rhizobacteria and its application prospects[J]. Chinese Journal of Applied Ecology, 2004, 15(10): 1963-1966.
[25]  Kang Y J, Shen M, Wang H L, et al. Effects of two plant growth-promoting rhizobacteria(PGPR) on yardlong bean early seedlings growth and indigenous soil bacterial community[J]. Journal of Agro-Environment Science, 2012, 31(8): 1537-1543.
[26]  Liu J L, Fang F, Shi X H, et al. Isolation and characterization of PGPR from the rhizosphere of the Avena sativa in saline-alkali soil[J]. Acta Prataculturae Sinica, 2013, 22(2): 132-139.
[27]  Yao T, Pu X P, Zhang D G, et al. Associative nitrogen-fixing bacteria in the rhizosphere of Avena sativa in an alpine region Ⅲ Effect on Avena sativa growth and quantification of nitrogen fixed[J]. Acta Prataculturae Sinica, 2004, 13(5): 101-105.
[28]  Zhang Y, Zhu Y, Yao T, et al. Interactions of four PGPRs isolated from pasture rhizosphere[J]. Acta Prataculturae Sinica, 2013, 22(1): 29-37.
[29]  Han H W, Sun L N, Yao T, et al. Effects of bio-fertilizers with different PGPR strain combinations on yield and quality of alfalfa[J]. Acta Prataculturae Sinica, 2013, 22(5): 104-112.
[30]  Wang N, Qin Y. Effects of AM fungus on root morphology of host plant bidens pilosa L.[J]. Journal of Anhui Agricultural Sciences, 2012, 40(1): 13-14.
[31]  Feng L, Zhang L H, Tian X S. Effect of pseudomonas flurosecens on rhizosphere microorganisms and root activity of tobacco[J]. Journal of Agro-Environment Science, 2007, 26(Supplement): 537-539.
[32]  Bonser A, Lynchj P, Snapp S. Effect of Phosphorus deficiency on growth angle of basal roots in Phaseolus vulgaris[J]. New Phytologist, 1996, 132: 281-288.
[33]  Zhao H, Xu F S, Shi L. Advances in plant root morphology adaptability to phosphorus deficiency stress[J]. Chinese Bulletin of Botany, 2006, 23(4): 409-417.
[34]  参考文献:
[35]  Adesemoye A O, Torbert H A, Kloepper J W. Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system[J]. Canadian Journal of Microbiology, 2008, 54(10): 876-886.
[36]  Oliveira C A, Alves V M C, Marriel I E. Phosphate-solubilizing microorganisms isolated from rhizosphere of maize cultivated in an oxisol of the Brazilian Cerrado Biome[J]. Soil Biology and Biochemistry, 2009, 41(9): 1782-1787.
[37]  Chen Z X, Ma S W, Liu L. Studies on phosphorus solubilizing activity of a strain of phosphor-bacteria isolated from chestnut type soil in China[J]. Bioresource Technology, 2008, 99(14): 6702-6707.
[38]  Hafeez F Y, Yasmin S, Ariani D, et al. Plant growth-promotingbacteria as biofertilizer[J]. Agronomy for Sustainable Development, 2006, 26: 143-150.
[39]  Suneja P, Dudeja S S, Narula N. Development of multiple co-inoculants of different biofertilizers and their interaction with plants[J]. Archives of Agronomy and Soil Science, 2007, 53(2): 221-230.
[40]  陈哲忠, 周省善. 333/A 春箭筈豌豆的选育与推广[M]. 兰州: 甘肃民族出版社, 1991.
[41]  王栋, 任继周. 牧草学各论[M]. 南京: 江苏科学技术出版社, 1989.
[42]  李琪. 高寒牧区箭豌豆与燕麦混播实验[J]. 中国草原与牧草, 1984, 1(1): 38-41.
[43]  赵秋. 北方低产土壤实用绿肥作物栽培与利用[M]. 天津: 天津科技翻译出版公司, 2010.
[44]  曾秀成, 王文明, 罗敏娜, 等. 缺素培养对大豆营养生长和根系形态的影响[J]. 植物营养与肥料学报, 2010, 16(4): 1032-1036.
[45]  王树起, 韩晓增, 严君, 等. 缺磷胁迫对大豆根系形态和氮磷吸收积累的影响[J]. 土壤通报, 2010, 41(3): 644-649.
[46]  丁红, 张智猛, 戴良香, 等. 不同抗旱性花生品种的根系形态发育及其对干旱胁迫的响应[J]. 生态学报, 2013, 33(17): 5169-5176.
[47]  刘霜, 李廷轩, 戢林, 等. 不同磷处理下两种生态型粗齿冷水花的富磷特征及根系形态差异[J]. 草业学报, 2013, 22(3): 211-217. 浏览
[48]  Hafeez F Y, Malik K A.Manual on Biofertilizer Technology[M]. Pakistan: NIBGE, 2000.
[49]  Malik K A, Bilal R. Survival and Colonization of Inoculated Bacteria in Kallar Grass Rhizosphere and Quantification of N2-Fixation[A]. In: Nitrogen Fixation with Nonlegumes[C]. Skinner F A, Bodderand R M, Fendrik I, (Eds). The Netherlands: Kluwer Academic Publishers, 1989: 301-310.
[50]  姚拓, 张德罡, 胡自治. 高寒地区燕麦根际联合固氮菌研究Ⅰ固氮菌分离及鉴定[J]. 草业学报, 2004, 13(2): 106-111.
[51]  姚拓. 高寒地区燕麦根际联合固氮菌研究Ⅱ固氮菌的溶磷性和分泌植物生长素特性测定[J]. 草业学报, 2004, 13(3): 85-90.
[52]  任大明, 张晓轩, 董丹, 等. 拮抗菌株Kc-t99的鉴定及其抑菌活性研究[J]. 生物技术, 2011, 4(4): 153-157.
[53]  Whiting S N, Neumann P M, Baker J M. Applying a solute transfer model to phytoextraction; Zinc acquisition by Thaspi caerulescens[J]. Plant Soil, 2003, 249: 45-56.
[54]  Hamdali H, Ouhdouch Y. Rock phosphate-solubilizing Actinomycetes Screening for plant growth-promoting activities[J]. World Journal of Microbiology and Biotechnology, 2008, 24: 2565-2575.
[55]  Mirz M S, Rasul G, Mehnaz S. Beneficial effects of inoculated nitrogen-fixing bacteria on rice[J]. Biology and Fertility of Soils, 2000, 31: 191-204.
[56]  Oliveira A M, Urquiaga S, Dobereiner J, et al. The effect of inoculating endophytic N2-fixing bacteria on micropropagated sugarcane plants[J]. Plant Soil, 2002, 242: 205-215.
[57]  胡江春, 薛德林, 马成新. 植物根际促生菌(PGPR)的研究与应用前景[J]. 应用生态学报, 2004, 15(10): 1963-1966.
[58]  康贻军, 沈敏, 王欢莉, 等. 两株PGPR对豇豆苗期生长及土著细菌群落的影响[J]. 农业环境科学学报, 2012, 31(8): 1537-1543.
[59]  刘佳莉, 方芳, 史煦涵, 等. 2株盐碱地燕麦根际促生菌的筛选及其促生作用研究[J]. 草业学报, 2013, 22(2): 132-139. 浏览
[60]  姚拓, 蒲小鹏, 张德罡, 等. 高寒地区燕麦根际联合固氮菌研究Ⅲ. 固氮菌对燕麦生长的影响及其固氮量测定[J]. 草业学报, 2004, 13(5): 101-105.
[61]  张英, 朱颖, 姚拓, 等. 分离自牧草根际四株促生菌株PGPR互作效应研究[J]. 草业学报, 2013, 22(1): 29-37. 浏览
[62]  韩华雯, 孙丽娜, 姚拓, 等. 不同促生菌株组合对紫花苜蓿产量和品质的影响[J]. 草业学报, 2013, 22(5): 104-112. 浏览
[63]  王宁, 秦艳. AM 真菌对宿主植物三叶鬼针草根系形态的影响[J]. 安徽农业科学, 2012, 40(1): 13-14.
[64]  冯莉, 张玲华, 田兴山. 荧光假单胞菌对烟草根际微生物种群数量及根系活力的影响[J]. 农业环境科学学报, 2007, 26(增刊): 537-539.
[65]  Bonser A, Lynchj P, Snapp S. Effect of Phosphorus deficiency on growth angle of basal roots in Phaseolus vulgaris[J]. New Phytologist, 1996, 132: 281-288.
[66]  赵华, 徐芳森, 石磊. 植物根系形态对低磷胁迫应答的研究进展[J]. 植物学通报, 2006, 23(4): 409-417.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133