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草业学报  2014 

苍耳提取物对萝卜蚜和粘虫作用方式及解毒酶活性影响的研究

DOI: 10.11686/cyxb20140434, PP. 276-284

Keywords: 苍耳,杀虫作用方式,解毒酶

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Abstract:

采用室内生测法,研究了苍耳3种溶剂(丙酮、乙醇、氯仿)提取物对萝卜蚜和粘虫的作用方式及苍耳氯仿提取物对两种试虫的3种解毒酶活性的影响。结果表明,苍耳氯仿提取物对萝卜蚜有很强的忌避作用,当浓度为0.05g/mL,处理4h,忌避率达94.25%,48h忌避率为84.53%;其次是触杀作用,校正死亡率最高为67.24%,LC50为0.7420g/mL;内吸作用极弱。对粘虫主要表现为拒食和生长发育抑制作用,拒食校正死亡率最高达98.27%,AFC50为0.1985g/mL;72h体重增长率为-5.85%,与对照差异极显著;胃毒和触杀毒力较弱,LC50分别为0.8997和1.3070g/mL。苍耳氯仿提取物对萝卜蚜和粘虫的乙酰胆碱酯酶(AchE)有抑制作用,处理萝卜蚜24h及处理粘虫4h时,乙酰胆碱酯酶(AchE)抑制率最高,分别为37.55%和25.65%;对谷胱甘肽转移酶(GSTs)和多功能氧化酶(MFO)活力影响不明显。

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