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草业学报  2014 

外源甜菜碱处理对干旱胁迫下半夏氮代谢及相关酶活性的影响

DOI: 10.11686/cyxb20140428, PP. 229-236

Keywords: 半夏,干旱胁迫,甜菜碱,氮代谢

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Abstract:

为探讨喷施外源甜菜碱溶液对干旱胁迫下半夏体内相关次生代谢产物及氮代谢相关酶活性的影响,采用盆栽控水法研究了外源甜菜碱对D(干旱处理,含水量35%±2%)、D20(干旱处理并加喷20mmol/L的甜菜碱)、D40(干旱处理并加喷40mmol/L的甜菜碱)各处理组半夏体内谷氨酸合酶(GOGAT)活性、谷氨酰胺合成酶(GS)活性、谷氨酸脱氢酶(GDH)活性、硝态氮、可溶性蛋白、总生物碱、鸟苷和腺苷含量的变化规律。结果表明随着干旱胁迫时间的增加,D40中GOGAT活性及GS活性降低幅度均小于D、D20,GDH活性呈先升高后降低的趋势,对照组(CK组,含水量75%±2%)变化不明显。在处理第9~27天时,D40与D20相比,总生物碱含量分别提高了6.3%,10.3%,12.3%,差异显著。腺苷和鸟苷含量随时间增加呈上升趋势,且D40含量均高于D20,CK组含量最高,腺苷在第27天时有降低趋势。在第27天时,D40可溶性蛋白含量分别比D、D20提高了7.3%和3.1%。表明40mmol/L的外源甜菜碱溶液能提高干旱胁迫条件下氮代谢相关酶的活性,从而加快无机氮的同化,有利于促进次生代谢物质的积累,同时也增强了半夏抵御干旱胁迫的能力。

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