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草业学报  2014 

不同早熟禾品种对干旱胁迫的生理响应及抗旱性评价

DOI: 10.11686/cyxb20140427, PP. 220-228

Keywords: 早熟禾,干旱胁迫,生理生化指标,苗期,PEG

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Abstract:

为鉴定评价早熟禾幼苗抗旱性,将14个早熟禾品种的种子经消毒后播种在塑料花盆中,出苗后间苗,2~3个真叶定株,每盆留生长一致、分布均匀的幼苗15株,定期浇水,以保证幼苗的正常生长。待生长到3~4个真叶时进行干旱处理,依据Michel公式计算不同水势所需的PEG-6000的量,然后加入PEG-6000溶液进行干旱处理,处理的水势梯度为0MPa(即为对照),-0.3,-0.6,-0.9和-1.2MPa。处理后每2d进行称重补水以维持溶液浓度。设干旱处理与对照(正常浇水)两组,对14个早熟禾品种幼苗进行模拟干旱胁迫,4次重复。测定叶绿素(CHL)含量、丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化物酶(POD)以及超氧化物歧化酶(SOD)活性等生理生化指标。结果表明,随着干旱胁迫的加剧,早熟禾幼苗叶片CHL含量呈下降趋势;Pro含量、MDA含量、SOD活性总体呈上升趋势;POD活性总体呈先降再升后降的趋势;利用隶属函数分析得出14个早熟禾品种的抗旱性强弱顺序依次为:3号恩托佩草地早熟禾>10号细叶早熟禾>1号优异草地早熟禾>2号斗士草地早熟禾=9号蓝钻草地早熟禾>7号草地早熟禾>11号草地早熟禾>4号草地早熟禾>5号瓦巴斯草地早熟禾>8号早熟禾=13号肯塔基草地早熟禾>14号草地早熟禾>6号莫诺波利草地早熟禾=12号菲尔津草地早熟禾。

References

[1]  郭逍宇, 熊薇, 宫辉力, 等. 冷季型草坪草对再生水灌溉响应及适宜性评价[J]. 环境科学与技术, 2011, 34(12H): 357-361. 
[2]  石永红, 万里强, 刘建宁, 等. 多年生黑麦草抗旱性主成分及隶属函数分析[J]. 草地学报, 2010, 18(5): 669-672. 
[3]  王彬, 李长鼎, 兰剑. 聚乙二醇-6000胁迫下十二个草地早熟禾品种萌发期抗旱性比较研究[J]. 北方园艺, 2012, 24: 54-58. 
[4]  穆怀彬, 伏兵哲, 德英. PEG-6000胁迫下10个苜蓿品种幼苗期抗旱性比较[J]. 草业科学, 2011, 28(10): 1809-1814. 
[5]  陈郡雯, 吴卫, 郑有良, 等. 聚乙二醇(PEG-6000)模拟干旱条件下白芷苗期抗旱性研究[J]. 中国中药杂志, 2010, 35(2): 149-153. 
[6]  李显利, 米福贵, 闫立军, 等. 草地早熟禾不同品种抗旱性的评价分析[J]. 草原与草坪, 2010, 30(3): 43-46. 
[7]  王彬, 李长鼎, 马仲泽, 等. 4个高羊茅品种幼苗期抗旱性比较研究[J]. 农业科学研究, 2011, 32(3): 22-26. 
[8]  张永峰, 殷波. 混合盐碱胁迫对苗期紫花苜蓿抗氧化酶活性及丙二醛含量的影响[J]. 草业学报, 2009, 18(1): 46-50.  浏览
[9]  李朝周, 左丽萍, 李毅, 等. 两个海拔分布下红砂叶片对渗透胁迫的生理响应[J]. 草业学报, 2013, 22(1): 176-182.  浏览
[10]  马彦军, 马瑞, 曹致中, 等. PEG胁迫对胡枝子幼苗叶片生理特性的影响[J]. 中国沙漠, 2012, 32(6): 1662-1668. 
[11]  何玮, 蒋安, 王琳, 等. PEG干旱胁迫对红三叶抗性生理生化指标的影响研究[J]. 中国农学通报, 2013, 29(5): 5-10. 
[12]  李州, 彭燕, 苏星源. 不同叶型白三叶抗氧化保护及渗透调节生理对干旱胁迫的响应[J]. 草业学报, 2013, 22(2): 257-263.  浏览
[13]  张昌胜, 刘国彬, 薛萐, 等. 浓度和CO2浓度升高条件下白羊草的光合特征[J]. 应用生态学报, 2012, 23(11): 3009-3015. 
[14]  籍越, 王德勤, 王爱武. 干旱胁迫对不同草坪草生理特性的影响[J]. 安徽农学通报, 2009, 15(20): 25-26. 
[15]  石永红, 万里强, 刘建宁, 等. 干旱胁迫对6个坪用多年生黑麦草品种抗旱性的影响[J]. 草地学报, 2009, 17(1): 52-57. 
[16]  冉秀芝. 冷季型草坪草抗热抗旱生理生化机理研究[D]. 重庆: 西南农业大学, 2013. 
[17]  杨顺强, 杨改河, 任广鑫, 等. 5种引进禾本科牧草抗旱性与抗寒性比较[J]. 西北农业学报, 2010, 19(4): 91-95. 
[18]  王志泰, 马瑞, 马彦军, 等. 利用隶属函数法分析胡枝子抗旱性[J]. 干旱区资源与环境, 2013, 27(9): 119-123.
[19]  陈雅君, 冯淑华, 陈桂芬, 等. 植物抗旱性鉴定指标的研究现状与进展[J]. 中国林副特产, 2005, 6: 26.
[20]  Reference:
[21]  Wan K J, Wu G L, Shi X X, et al. Advances in the research of the responses and adjustment of turf-grass to drought stress[J]. Pratacultural Science, 2006, 23(8): 97-102. 
[22]  Levit J. Response of Plant to Environmental Stresses[M]. New York: Academic Press, 1972. 
[23]  Turner N C. Adaptation to water deficits: A changing perspective[J]. Australian Journal of Plant Physiology, 1983, 13: 175-190. 
[24]  Sun T J, Su R G G, Ma W L, et al. Drought resistance of ten seedling grasses[J]. Acta Prataculturae Sinica, 2008, 17(4): 42-49. 
[25]  Xu G F. Effects of PEG stress on resistance physiological and biochemical indexes of adversity of two Lysimachia species[J]. Acta Prataculturae Sinica, 2008, 17(1): 66-70.
[26]  Wang J P, Bughrara S S. Evaluation of drought tolerance for Atlas fescue, perennial ryegrass, and their progeny[J]. Euphytica, 2008, 164: 113-122. 
[27]  Abraham E M, Huang B R, Bonos S A, et al. Evaluation of drought resistance for Texas bluegrass, Kentucky bluegrass, and their hybrids[J]. Crop Science, 2004, 44: 1746-1753. 
[28]  Michcl B E, Kaufmann M R. The osmotic potential of polyethylene glycol 6000[J]. Plant Physiology, 1973, 51: 914-916. 
[29]  Li H S. Plant Physiology and Biochemistry experimental principles and techniques[M]. Beijing: Higher Education Press, 2000. 
[30]  Zhang Z L. Plant physiology test guideline[M]. Beijing: Higher Education Press, 1990: 305-312. 
[31]  Su G X, Hong F S. Evaluation of salt tolerance for partial mulberry varieties with subordinate function[J]. Jiangsu Journal of Agricultural Sciences, 2002, 18(1): 42-47. 
[32]  Jiao X J, Dong L H, Ding C B, et al. Effects of drought stress on reactive oxygen species and their scavenging systems in Chlorophytum capense var.medio-pictum leaf[J]. Acta Prataculturae Sinica, 2013, 22(5): 248-255. 
[33]  Lu C H. Study on drought resistance and evaluation on turf use quality of eight wild annual bluegrass[D]. Lanzhou: Gansu Agricultural University, 2010. 
[34]  Wang L Q, Shi Y H, Li X L, et al. Physiological resistance of three Lolium perenne L. varieties under PEG stress[J]. Acta Agrectir Sinica,2009, 17(4): 440-441. 
[35]  Shan N W, Jin J S, Zhao X T, et al. Relationship between the tolerance to water deficit stress and superoxide dismutases (SODs) in petal of cut rose Samantha[J]. Scientia Agricultura Sinica, 2005, 38(7): 1431-1438. 
[36]  Guo X Y, Xiong W, Gong H L, et al. Effects of reclaimed water irrigation on characteristics of physiology in three cool-season grasses and their feasibility[J]. Environmental Science & Technology, 2011, 34(12H): 357-361. 
[37]  Shi Y H, Wan L Q, Liu J N, et al. Analysis of the principal components and the subordinate function of Lolium perenne drought resistance[J]. Acta Agrictir Sinica, 2010, 18(5): 669-672. 
[38]  Wang B, Li C D, Lan J. Study on drought resistance at germination stage of 12 varieties of Poa pratensis L.under PEG-6000 stress[J]. Northern Horticulture, 2012, 24: 54-58. 
[39]  Mu H B, Fu B Z, De Y. Drought tolerance of alfalfa seedlings of 10 varieties under PEG-6000 stress[J]. Pratacultural Science, 2011, 28(10): 1809-1814. 
[40]  Chen J W, Wu W, Zheng Y L, et al. Drought resistance of Angelica dahurica during seedling stage under polyethylene glycol (PEG-6000)-simulated drought stress[J]. China Journal of Chinese Materia Medica, 2010, 35(2): 149-153. 
[41]  Li X L, Mi F G, Yan L J, et al. Evaluation and analysis of the drought resistance of different bluegrass varieties[J]. Grassland and Turf, 2010, 30(3): 43-46. 
[42]  Wang B, Li C D, Ma Z Z, et al. Study on drought resistance at seedling stage of 4 varieties of tall fescue[J]. Journal of Agricultural Sciences, 2011, 32(3): 22-26. 
[43]  Zhang Y F, Yin B. Influences of salt and alkali mixed stresses on antioxidative activity and MDA content of Medicago sativa at seedling stage[J]. Acta Prataculturae Sinica,2009, 18(1): 46-50. 
[44]  Li C Z, Zuo L P, Li Y, et al. Physiological responses in leaves of Reaumuria soongorica from different altitudes under osmotic stress[J]. Acta Prataculturae Sinica, 2013, 22(1): 176-182. 
[45]  Ma Y J, Ma R, Cao Z Z, et al. Effects of PEG stress on physiological characteristics of the Lespedeza seedlings leaves[J]. Journal of Desert Research, 2012, 32(6): 1662-1668. 
[46]  He W, Jiang A, Wang L, et al. Study on the effects of PEG stress on physiological and biochemical indexes of five red clovers[J]. Chinese Agricultural Science Bulletin, 2013, 29(5): 5-10. 
[47]  Li Z, Peng Y, Su X Y. Physiological responses of white clover by different leaf types associated with anti-oxidative enzyme protection and osmotic adjustment under drought stress[J]. Acta Prataculturae Sinica, 2013, 22(2): 257-263. 
[48]  Zhang C S, Liu G B, Xue S, et al.Photosyntheticcharacteristicsof Bothriochloa ischaemum under drought stress andelevated CO2 concentration[J]. Chinese Journal of Applied Ecology, 2012, 23(11): 3009-3015. 
[49]  Ji Y, Wang D Q, Wang A W. Effects of drought stress on physiological character in various lawn breeds[J]. Anhui Agricultural Science Bulletin, 2009, 15(20): 25-26. 
[50]  Shi Y H, Wan L Q, Liu J N, et al. Effects of PEG stress on the drought resistance of six turfgrass varieties of Lolium perenne L.[J]. Acta Agrectir Sinica, 2009, 17(1): 52-57. 
[51]  Ran X Z. Studies on physiological and biochemical mechanisms of heat-resistance and drought-tesistance of cool-season turfgrasses[D]. Chongqing: Southwest Agricultural University, 2013. 
[52]  Yang S Q, Yang G H, Ren G X, et al. A comparative study on drought resistance and frost resistance of five kinds of introduced grasses[J]. Acta Agriculturae Boresli-Occidentalis Sinica, 2010, 19(4): 91-95. 
[53]  Wang Z T, Ma R, Ma Y J, et al. The drought resistance of 5 species of Lespedeza[J]. Journal of Arid Land Resources and Environment, 2013, 27(9): 119-123.
[54]  Chen Y J, Feng S H, Chen G F, et al. Current researches of the evaluation indicators of anti-drought plant agents[J]. Forest By-Product and Speciality in China, 2005, 6: 26.
[55]  参考文献:
[56]  万开军, 武高林, 史晓霞, 等. 草坪草对干旱胁迫的反应与调节研究进展[J]. 草业科学, 2006, 23(8): 97-102. 
[57]  Levit J. Response of Plant to Environmental Stresses[M]. New York: Academic Press, 1972. 
[58]  Turner N C. Adaptation to water deficits: A changing perspective[J]. Australian Journal of Plant Physiology, 1983, 13: 175-190. 
[59]  孙铁军, 苏日古嘎, 马万里, 等. 10种禾草苗期抗旱性的比较研究[J]. 草业学报, 2008, 17(4): 42-49. 
[60]  许桂芳. PEG胁迫对2种过路黄抗性生理生化指标的影响[J]. 草业学报, 2008, 17(1): 66-70. 
[61]  Wang J P, Bughrara S S. Evaluation of drought tolerance for Atlas fescue, perennial ryegrass, and their progeny[J]. Euphytica, 2008, 164: 113-122. 
[62]  Abraham E M, Huang B R, Bonos S A, et al. Evaluation of drought resistance for Texas bluegrass, Kentucky bluegrass, and their hybrids[J]. Crop Science, 2004, 44: 1746-1753. 
[63]  Michcl B E, Kaufmann M R. The osmotic potential of polyethylene glycol 6000[J]. Plant Physiology, 1973, 51: 914-916. 
[64]  李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000. 
[65]  张志良. 植物生理学试验指导[M]. 北京: 高等教育出版社, 1990: 305-312. 
[66]  苏国兴, 洪法水. 桑品种耐盐性的隶属函数法之评价[J]. 江苏农业学报, 2002, 18(1): 42-47. 
[67]  贾学静, 董立花, 丁春邦, 等. 干旱胁迫对金心吊兰叶片活性氧及其清除系统的影响[J]. 草业学报, 2013, 22(5): 248-255.  浏览
[68]  鲁存海. 8种野生早熟禾抗旱性及草坪质量综合评价研究[D]. 兰州: 甘肃农业大学, 2010. 
[69]  万里强, 石永红, 李向林, 等. PEG胁迫下3个多年生黑麦草品种抗性生理研究[J]. 草地学报, 2009, 17(4): 440-441. 
[70]  单宁伟, 金基石, 赵喜亭, 等. 切花月季Samantha失水胁迫耐性与其超氧化物歧化酶之间的关联[J]. 中国农业科学, 2005, 38(7): 1431-1438. 

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