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草业学报  2014 

NaCl处理对梭梭生长及生理生态特征的影响

DOI: 10.11686/cyxb20140317, PP. 152-159

Keywords: 梭梭,盐胁迫,MDA含量,抗氧化系统,渗透调节

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Abstract:

以1年生梭梭幼株为材料,采用盆栽试验研究不同浓度(0,50,100,200,400mmol/L)NaCl处理对梭梭生长状况及同化枝过氧化氢(H2O2)、丙二醛(MDA)含量、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)活性、水势、可溶性糖和脯氨酸含量的影响。结果表明,低浓度的NaCl处理(50mmol/L)对梭梭的生长具有促进作用,梭梭株高和基茎粗在400mmol/LNaCl处理下较对照显著减少,冠幅面积和同化枝、茎干重在NaCl浓度为≥200mmol/L时较对照开始显著减少,根干重在NaCl浓度为≥100mmol/L时较对照呈显著减少趋势。因此,NaCl胁迫对梭梭根生长的抑制作用大于对冠幅面积,同化枝和茎干重大于对株高和基茎粗。根冠比在NaCl浓度≥100mmol/L时较对照显著减少。H2O2和MDA含量在高浓度NaCl处理下较对照出现积累现象。低浓度NaCl处理下(≤100mmol/L),梭梭同化枝SOD、POD、CAT和APX活性较对照均有所提高,它们协调一致的共同作用,有效地清除植物体内的活性氧(reactiveoxygenspecies,ROS),高浓度的NaCl处理下(≥200mmol/L),CAT和POD活性较对照开始降低,抗氧化酶系统对ROS的清除效率减弱。梭梭同化枝水势随NaCl处理浓度升高而降低。可溶性糖和脯氨酸含量随NaCl浓度增加较对照先上升后降低。

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