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草业学报  2014 

马铃薯贮藏期主要病害拮抗内生细菌的筛选、鉴定及功能评价

DOI: 10.11686/cyxb20140332, PP. 269-275

Keywords: 内生细菌,16SrDNA鉴定,拮抗作用,生物学功能

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Abstract:

马铃薯坏疽病、炭疽病和枯萎病是甘肃省马铃薯贮藏期最主要的病害,为了获得马铃薯贮藏期病害防治的生防菌株,利用对峙培养法从东祁连山高寒草地线叶嵩草内生细菌中筛选获得高抗菌株263XY1,其对马铃薯枯萎病菌、马铃薯炭疽病菌和马铃薯坏疽病菌的抑菌率分别达到67.17%,86.17%和70.89%;该菌株在含有色氨酸和不含色氨酸的金氏培养基中分泌的IAA分别为3.31和1.52mg/L,在PKO含无机磷培养基中的有效磷增量为38.94mg/L,具有固氮能力。经16SrDNA序列分析,其与死谷芽孢杆菌(Bacillusvallismortis,登录号:JQ547633.1)同源性达99%,初步鉴定为死谷芽孢杆菌。

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