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草业学报  2014 

低氢氰酸含量高丹草新品系及其亲本的SSR分析

DOI: 10.11686/cyxb20140127, PP. 223-228

Keywords: 高丹草,低氢氰酸含量,新品系,SSR分析,指纹图

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Abstract:

为明确4个低氢氰酸含量的高丹草新品系SLCN-11、SLCN-12、SLCN-13、SLCN-14及其亲本散穗高粱、黑壳苏丹草、白壳苏丹草、红壳苏丹草和棕壳苏丹草在DNA水平上的差异程度,以育成登记的高丹草品种蒙农青饲3号为对照,对其进行了SSR分析。试验从200个高粱SSR引物中筛选出多态性丰富、重复性好的12对引物,利用这些引物对10个材料进行PCR扩增共得到509个多态性位点,多态性百分率达87.30%。每对引物扩增的SSR指纹图清晰、稳定,可作为鉴别4个高丹草新品系及其亲本的分子依据。10个供试材料间的遗传距离(GD)变幅在0.3165~0.6692之间,平均为0.5359;以GD值0.50为基准,将10个材料划分为5类:蒙农青饲3号、散穗高粱、SLCN-11、SLCN-12、SLCN-13为一类;SLCN-14、棕壳苏丹草为一类;白壳苏丹草、黑壳苏丹草、红壳苏丹草各单独为一类。该研究为下一步低氢氰酸含量高丹草新品种育成及登记利用奠定了基础。

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