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草业学报  2014 

山西白羊草种质资源遗传多样性的ISSR分析

DOI: 10.11686/cyxb20140126, PP. 217-222

Keywords: 白羊草,ISSR,遗传多样性,山西,无融合生殖

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Abstract:

利用ISSR分子标记技术对山西33个白羊草种质资源进行遗传多样性分析。白羊草种质间遗传变异较稳定,10条ISSR引物共扩增出99条带,多态性比率(P)为52.23%;通过PopGen32软件计算出:等位基因数(Na)为1.5253±0.5019,有效等位基因数(Ne)为1.2941±0.3665,Nei’s基因多样性(H)为0.1723±0.1983,Shannon信息指数(I)为0.2590±0.2841;通过NTSYSpc1.20c软件计算出33份材料间的遗传相似系数(GS)为0.8188~0.9801,遗传距离(GD)为0.0200~0.1988。结果表明,33份种质材料居群水平的遗传多样性较低,而自然条件下白羊草进行无融合生殖可能是导致白羊草居群遗传多样性较低的原因之一。

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