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草业学报  2014 

黄花蒿抗疟相关成分及抗氧化活性对施肥方式的响应

DOI: 10.11686/cyxb20140140, PP. 339-345

Keywords: 黄花蒿,施肥,青蒿酸,去氧青蒿素,青蒿素,总黄酮,抗氧化活性

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Abstract:

本研究探讨施肥对黄花蒿抗疟相关成分及抗氧化活性的影响,为科学施肥和提高黄花蒿产量品质提供理论依据。采用盆栽试验,设置不同施肥处理,于黄花蒿现蕾期分别采样测定根、茎、叶中的青蒿酸、去氧青蒿素、青蒿素、总黄酮含量及累积量,分析各器官乙醇提取液对DPPH·的清除率。结果表明,施肥显著促进黄花蒿生长,单株生物产量分别比不施肥增加57.4%(无机肥)、91.6%(有机肥)和92.3%(有机无机配施)。在黄花蒿体内,青蒿酸、去氧青蒿素、青蒿素、总黄酮的含量和累积量高低趋势均表现为:叶>茎>根,说明叶片是这些物质合成和储存的主要器官。此外,叶片的抗氧化活性(DPPH·清除率)也最高,显著高于根系和茎,并且与总黄酮含量呈显著正相关。DPPH·清除率总体为不施肥处理最高,施肥后不同程度降低,施肥对总黄酮含量影响不大,但显著提高了青蒿酸、去氧青蒿素、青蒿素的含量和累积量(产量)及总黄酮的产量,其中有机无机配施和有机肥处理总体优于无机肥。说明在集约化种植黄花蒿的过程中,提倡施用有机肥是必要的。

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