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草业学报  2015 

紫花苜蓿根腐病原菌——拟枝孢镰刀菌的鉴定及其生物学特性研究

DOI: 10.11686/cyxb2014466, PP. 88-98

Keywords: 紫花苜蓿,拟枝孢镰刀菌,病原鉴定,生物学特性

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Abstract:

本文对从内蒙古赤峰巴雅尔草业基地紫花苜蓿根部分离到的菌株BYE27-2-5进行了致病性测定、形态学和rDNA-ITS序列分析鉴定及生物学特性测定。结果表明,该菌菌丝生长、产孢和孢子萌发的最适温度分别为25,30和28℃;pH值5.0~11.0均宜于菌丝生长,产孢最适pH8.0,孢子萌发最适pH7.0;光暗交替利于菌丝生长,光照利于产孢和孢子的萌发;孢子在相对湿度低于75%不萌发,高于95%萌发较快;葡萄糖蛋白胨培养基利于菌丝生长和孢子萌发,马铃薯蔗糖琼脂培养基利于产孢;碳源中葡萄糖利于菌丝生长和孢子萌发,蔗糖利于产孢;氮源中蛋白胨利于菌丝生长,酵母膏利于产孢和孢子萌发;菌丝致死温度为54℃(10min),分生孢子的致死温度为48℃(10min);根据其形态特征和rDNA-ITS序列分析结果,鉴定其为拟枝孢镰刀菌(Fusariumsporotrichioide)。

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