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草业学报  2015 

铅胁迫对禾本科牧草生长、生理及Pb2+富集转运的影响

DOI: 10.11686/cyxb2014303, PP. 163-172

Keywords: 禾本科牧草,苗期,铅胁迫,生长和生理,富集转运

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Abstract:

采用苗期盆栽试验,对14份禾本科牧草种质材料进行不同浓度(0,10,50,100,200,300mg/kg)铅(Pb)胁迫处理,通过对株高、分蘖数、地上/地下生物量、脯氨酸含量、抗氧化酶(SOD、POD、CAT)活性、地上部与根中Pb2+含量等指标的测定与分析,探究Pb胁迫对禾草生长生理以及Pb2+富集转运的影响。结果表明,随着Pb浓度的增加,14份材料的株高、分蘖数、地上生物量均呈先上升后下降的趋势,地下生物量则逐渐降低,通过综合评价,来自土耳其的长穗冰草G5具有较强的耐Pb性。随着Pb浓度的增加,地上部与根中Pb2+含量逐渐升高,富集系数逐渐降低,转移系数逐渐降低(披碱草G7与G8除外),根中Pb2+含量显著高于地上部,为Pb2+的主要富集器官,披碱草地上部Pb2+含量高于其他材料,具有较强的Pb富集转运能力。7份材料的生理指标测定结果表明,随着Pb浓度的增加,7份材料的脯氨酸含量逐渐上升,CAT活性逐渐下降,POD和SOD活性则呈先上升后下降的趋势。

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