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草业学报  2015 

中国西北地区披碱草属植物所带内生真菌的培养特征

DOI: 10.11686/cyxb2014411, PP. 89-95

Keywords: 内生真菌,披碱草,菌落,多样性,系统发育

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Abstract:

内生真菌与植物所形成的共生体普遍存在于冷季禾草中,共生体有利于提高宿主植物的生物和非生物抗性。虽然目前研究者们已对禾草内生真菌做了大量的研究,但是尚未发现对分布于中国西北地区披碱草属植物无性世代Neotyphodium内生真菌培养特征的研究。本研究从中国西北地区分离出15株来自不同地理种群披碱草属植物无性世代Neotyphodium内生真菌。通过分离培养,测定了这15株无性世代Neotyphodium内生真菌的菌落生长速度,结果显示,这15株无性世代Neotyphodium内生真菌菌落的平均生长速度为(0.41~0.85)mm/d,且生长速度与海拔具有相关性。分布在海拔低于3000m的无性世代Neotyphodium内生真菌的菌落生长速度比生长于海拔高于3000m菌落的生长速度快。观察菌落发现,分布在海拔高于3000m的菌落正面特征较为一致,而分布在海拔低于3000m的菌落正面特征出现不同。另外,通过多基因联合建树发现,分布在海拔高于3000m的无性世代Neotyphodium内生真菌聚在一个分支,而分布在海拔低于3000m的无性世代Neotyphodium内生真菌呈现星状分布。由于目前尚未对中国西北地区披碱草属植物无性世代Neotyphodium内生真菌命名。因此,希望本研究结果能为该菌的命名提供理论依据。

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