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草业学报  2015 

外源一氧化氮提高裸燕麦幼苗的耐碱性

DOI: 10.11686/cyxb2014352, PP. 110-117

Keywords: 一氧化氮,裸燕麦,碱胁迫,活性氧代谢,渗透溶质

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Abstract:

采用营养液砂培方法,研究外源一氧化氮(NO)供体硝普钠(SNP)对NaHCO3胁迫下裸燕麦幼苗生长、活性氧代谢和渗透溶质积累的影响。结果表明,1~200μmol/LSNP能够缓解75mmol/LNaHCO3胁迫对裸燕麦幼苗生长的抑制作用,25μmol/LSNP的缓解作用最明显,可降低裸燕麦叶片O2·-、H2O2、丙二醛和有机酸含量,增强幼苗叶片超氧化物歧化酶、过氧化氢酶、过氧化物酶、抗坏血酸过氧化物酶和质膜H+-ATPase活性,提高叶片谷胱甘肽、可溶性糖、可溶性蛋白质、游离氨基酸、脯氨酸含量和K+/Na+,但对抗坏血酸含量影响不大。分析表明,外源NO可能通过激活抗氧化系统活性、促进渗透溶质积累和改善Na+、K+平衡缓解碱胁迫对幼苗的伤害和生长抑制,从而提高裸燕麦的耐碱性。

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