全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用:99美元

查看量下载量

相关文章

更多...
草业学报  2015 

GA3和CEPA对香水百合开花期光合生理和抗氧化酶活性的影响及其花期响应研究

DOI: 10.11686/cyxb2015041, PP. 73-84

Keywords: 赤霉素,乙烯利,花期,光合特性,抗氧化酶

Full-Text   Cite this paper   Add to My Lib

Abstract:

以香水百合为材料,采用盆栽试验,研究了在不同浓度赤霉素(GA3)、乙烯利(CEPA)的处理影响下,香水百合的花期(现蕾期、初花期、盛花期和末花期)变化、光合生理和抗氧化酶活性的变化及其花期响应。结果表明,1)在香水百合开花期,GA3和CEPA处理均不同程度地提高了香水百合株高、茎粗、蕾长和花径,并提前了初花期,延长了开花时间。GA3与株高、茎粗和蕾长呈显著正相关,对开花前中期影响显著,而CEPA与茎粗、蕾长和花径呈显著正相关,与末花天数呈显著负相关;2)GA3和CEPA处理提高了各时期光合色素含量,并有效缓解了末花期叶绿素的下降趋势,延长了光合作用时间;3)GA3和CEPA处理显著提高了香水百合开花期Pn、Gs、Tr和Ls,而Ci和LUE与其他参数呈负相关关系,分析表明,GA3和CEPA处理增强了花期叶片细胞的活性,提高了叶片的光合作用强度;4)GA3和CEPA处理的百合叶片MDA含量在开花各时期显著低于对照,抗氧化酶SOD、CAT活性上升,同时,处理减缓了开花中后期SOD下降趋势,有效缓解了衰老进程,达到延长香水百合花期的目的。综合分析表明,GA3和CEPA提高了香水百合开花各时期的光合特性,并增强了抗氧化酶活性,进而对香水百合开花品质和开花花期有显著影响。其中,GA3200mg/L+CEPA30mg/L协同处理对提前花期和延长开花持续天数的调节效果最佳,其次为单水平GA3200mg/L处理和单水平CEPA60mg/L处理,高浓度GA3200mg/L+CEPA60mg/L和GA3300mg/L+CEPA30mg/L的调节作用下降。

References

[1]  Ren Y F, He J Y, Wang S M, et al . Changes of anti-oxidant enzymes activities during florescence and flower senescence of navel orange. Chinese Agricultural Science Bulletin, 2009, 25(19): 62-64.
[2]  Li Y H, Wan G H, Ye Q S. Studies on photosynthetic characteristics of Gerbera hybrida var. diablo . Subtropical Plant Science, 2005, 34(1): 14-17.
[3]  Zhou Y L. The Role of the Endogenous Anti-oxidant System on Flower Opening and Senescence Process in Tree Peony[D]. Beijing: Beijing Forestry University, 2010.
[4]  Zheng D F, Zhao L M, Feng N J. Effects of plant growth regulators (PGRs) on endogenous hormone contents and activities of protective enzymes in soybean leaves. Acta Agronomica Sinica, 2008, 34(7): 1233-1239.
[5]  Jia H S, Lu C M. Effects of abscisic acid on photo inhibition in maize plants. Plant Science, 2003, 165: 1403-1410.
[6]  Huang Z Z. The Flowering Regulation of Flowers[M]. Beijing: Chinese Forestry Press, 1990: 33-41.
[7]  Huang Z H. Study on the Effects of GA 3 on the Photosynthetic Characteristics and Growth of Tissue-cultured Derived Plant Lily[D]. Guilin: Guangxi University, 2006.
[8]  Zhu Q. Effects of Different N,P,K,Ca Application Levels on Plant Growth and Cut Flower Quality of Lilium casa blanca [D]. Chengdu: Sichuan Agricultural University, 2012.
[9]  Peng G Q, Wang L H. Quantitative variation of endogenous hormones in Rosa rugosa leaf during flower bud differentiation. Bulletin of Botanical Research, 2006, 26(2): 206-210.
[10]  Zhao J Y, Pan Y Z, Li Y H. Effects of abscisic acid on flowering and some key enzymes of Bougainvillea glabra . Acta Horticulturae Sinica, 2014, 41(10): 2085-2093.
[11]  Gao J F. Experimental Guidance for Plant Physiology[M]. Beijing: Higher Education Press, 2006.
[12]  Xiong Q E. Plant Physiology[M]. Chengdu: Sichuan Science and Technology Press, 2003.
[13]  Li H S. Principle and Technology of Plant Physiological and Biochemical Experiment[M]. Beijing: Higher Education Press, 2000.
[14]  Cai X M, Lin Z, Zheng D J, et al . The study of chemical regulation on florescence of Lilium oriental “Sorbonne”. Chinese Agricultural Science Bulletin, 2006, 22(6): 280-282.
[15]  Shen H J, Huang Z X, Gui R Y. Studies on regulation of flower bud differentiation of Lilium longiflorum , Cyclamen persicum and Senecio cruentus . Journal of Nanjing Forestry University, 2001, 25(2): 55-57.
[16]  龙亚宜.百合—球根花卉之王[M]. 北京: 京盾出版社, 1999: 5.
[17]  陈诗林, 黄敏玲. 低温和赤霉素对亚洲百合开花及鳞茎繁殖的效应. 吉林农业大学学报, 2007, 29(5): 511-517.
[18]  赵莉, 潘远智, 朱峤, 等. 6-BA、GA 3 和IBA对香水百合叶绿素含量及抗氧化酶活性的影响. 草业学报, 2012, 21(5): 248-256.
[19]  任艳芳, 何俊瑜, 王思梦, 等. 脐橙花开放和衰老过程中抗氧化酶活性变化. 中国农学通报, 2009, 25(19): 62-64.
[20]  李永华, 万国辉, 叶庆生. 非洲菊光合特性研究. 亚热带植物科学, 2005, 34(1): 14-17.
[21]  周昕蕾. 内源抗氧化酶在牡丹开放和衰老进程中作用的研究[D]. 北京: 北京林业大学, 2010.
[22]  郑殿峰, 赵黎明, 冯乃杰. 植物生长调节剂对大豆叶片内源激素含量及保护酶活性的影响. 作物学报, 2008, 34(7): 1233-1239.
[23]  黄章智. 花卉的花期调节[M]. 北京: 中国林业出版社, 1990: 33-41.
[24]  黄樟华. 赤霉素对百合组培苗光合特性和生长状况的影响研究[D]. 桂林:广西大学, 2006.
[25]  朱峤. 不同氮、磷、钾、钙水平对香水百合生长发育和切花品质效应的研究[D]. 成都: 四川农业大学, 2012.
[26]  彭桂群, 王力华.平阴玫瑰花芽分化期叶片内源激素的变化.植物研究, 2006, 26(2): 206-210.
[27]  赵家昱, 潘远智, 李永红. 外源ABA对叶子花开花及内源ABA合成关键酶的影响. 园艺学报, 2014, 41(10): 2085-2093.
[28]  高俊凤. 植物生理学实验指导[M]. 北京: 高等教育出版社, 2006.
[29]  熊庆娥. 植物生理学实验教程[M]. 成都: 四川科学技术出版社, 2003.
[30]  李合生. 植物生理生化实验原理和技术[M]. 北京: 高等教育出版社, 2000.
[31]  蔡宣梅, 林真, 郑大江, 等. 东方百合“Sorbonne”花期化学调控技术研究. 中国农学通报, 2006, 22(6): 280-282.
[32]  沈慧娟, 黄作喜, 桂仁意. 麝香百合、仙客来、瓜叶菊花芽分化的调控. 南京林业大学学报, 2001, 25(2): 55-57.
[33]  高俊平. 切花衰老与乙烯.园艺学年评[M]. 北京: 科学出版社, 1995.
[34]  张琳, 夏宜平, 杜方, 等. 乙烯与百合切花瓶插寿命的关系研究进展. 园艺学报, 2013, 40(9): 1826-1836.
[35]  任小林, 李海峰, 弓德强, 等. 秋施乙烯利和赤霉素对牡丹萌芽及开花的影响. 西北植物学报, 2004, 24(5): 895-898.
[36]  刘雅莉, 王飞, 黄森. 百合花朵不同发育期乙烯释放量与膜脂过氧化作用的研究. 西北植物学报, 1999, 19(6): 143-147.
[37]  李改丽, 张延龙, 牛立新. 植物生长调节剂TDZ对“索邦”百合果实生长发育的影响. 园艺学报, 2013, 40(2): 299-306.
[38]  孙小玲, 许岳飞, 马鲁沂, 等. 植株叶片的光合色素构成对遮阴的响应. 植物生态学报, 2010, 34(8): 989-999.
[39]  邹燕, 王瑞雪, 沈亮余, 等. 渗透胁迫下外源茉莉酸甲酯对油菜种子生理特性的影响. 西北植物学报, 2011, 31(3): 564-568.
[40]  孙凌霞, 孙萍, 蔡仕珍, 等. 四种冷季型草坪草对二氧化硫胁迫的生理响应研究. 草业学报, 2014, 23(4): 237-244. 浏览
[41]  Wang F Z, Tang J. Flora Republicae Popularis Sinicae(Tomus 14)[M]. Beijing: Science Press, 1980:116-166.
[42]  Chen R S. Flowering Bulbs[M]. Fuzhou: Fujian Science and Technology Public House, 2001.
[43]  Long Y Y. Lily: The King of the Bulb Flower[M]. Beijing: Jingdun Publishing House, 1999:5.
[44]  Blankenship S M, Bailey D A, Miller J E. Effects of continous low levels of ethylene on growth and flowering og Easter lily. Scientia Horticulturae, 1993, 53(4): 311-317.
[45]  Sodi A M, Panizza M. Tognoni F. Ethylene involvement in vitro regeneration of lily. Acta Horticulturae, 1990, 280: 151-154.
[46]  Elgar H J, Woolf A B, Bieleski R L. Ethylene production by three lily species and their response to ethylene exposure. Postharvest Biology and Technology, 2002, 16(3): 257-267.
[47]  Chen S L, Huang M L. Effects of chilling and gibberellin on flowering and bulb reproduction of asiatic hybrid lily. Journal of Jilin Agricultural University, 2007, 29(5): 511-517.
[48]  Zhao L, Pan Y Z, Zhu Q, et al . Effects of 6-BA, GA 3 and IBA on photosynthetic pigment content and related enzyme activities of Lilium casa blanca . Acta Prataculturae Sinica, 2012, 21(5): 248-256.
[49]  Gao J P. The role of ethylene in the senescence of cut flower. Annual Review of Gardening[M]. Beijing: Science Press, 1995.
[50]  Reid M S, Paul J L, Farhoomand M B, et al . Pulse treatments with the silver thiosulfate complex extend the vase life of cut carnations. Journal of the American Society for Horticultural Science, 1980, 105(1): 25-27.
[51]  Zhang L, Xia Y P, Du F, et al . Research advance in relationship between ethylene and vase life of cut lily flowers. Acta Horticulturae Sinica, 2013, 40(9): 1826-1836.
[52]  Ren X L, Li H F, Gong D Q, et al . Effects of application of ethephon and GA 3 in fall on bud sprouting and flowering of peony. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24(5):895-898.
[53]  Rood S B. Endogenous gibberellins and shoot growth and development in Brassica napus . Plant Physiology, 1989, 89:530-534.
[54]  Liu Y L, Wang F, Huang S. Study on the ethylene release and the membrantlipid peroxidation in the different development stage of lilium flower. Acta Botanica Boreali-Occidentalia Sinica, 1999, 19(6): 143-147.
[55]  Vavilin D, Vermaas W. Chlorophy B can serve as the major pigment in functional photosystem II complexes of cyanobacteria. Proceedings of the National Academy of Science, 2001, 98(24): 14168-14173.
[56]  Li G L, Zhang Y L, Niu L X. Effects of thidiazuron fruit growth and development lilium oriental hybrids ‘Sorbonne’. Acta Horticulturae Sinica, 2013, 40(2): 299-306.
[57]  Sun X L, Xu Y F, Ma L Y, et al . A review of acclimation of photosynthetic pigment composition in plant leaves to shade environment. Chinese Journal of Plant Ecology, 2010, 34(8): 989-999.
[58]  Farquhar G D, Sharkey T D. Stomatal conductance and photosynthesis. Annual Review of Plant Physiology, 1982, 33: 317-345.
[59]  Zou Y, Wang R X, Shen L Y, et al . Effects of exogenous meJA on physiological characteristics of Brassica napus under osmotic stress. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(3): 564-568.
[60]  Sun L X, Sun P, Cai S Z, et al . Physiological responses of four cold-season turfgrasses to oxidative stress of SO 2 . Acta Prataculturae Sinica, 2014, 23(4): 237-244.
[61]  Milone M T, Sgherri C, Clijsters H, et al . Antioxidative responses of wheat treated with realistic concentration of cadmium. Environmental and Experimental Botany, 2003, 50(3): 265-276.
[62]  汪发缵, 唐进. 中国植物志(第14卷)·百合科[M]. 北京: 科学出版社, 1980: 116-166.
[63]  陈榕生.球根花卉[M]. 福州: 福建科学技术出版社, 2001.

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133