全部 标题 作者
关键词 摘要

OALib Journal期刊
ISSN: 2333-9721
费用：99美元
投递稿件

查看量下载量

相关文章

更多...
草业学报  2015 

番茄细菌性叶斑病菌的拮抗菌筛选、鉴定及其拮抗性能评价

DOI: 10.11686/cyxb2014450, PP. 166-173

Keywords: 番茄叶斑病菌,拮抗内生细菌,筛选,鉴定,防效

Full-Text   Cite this paper   Add to My Lib

Abstract:

采用含菌平板抑菌圈测定法,从120株高寒草地牧草内生细菌中分离筛选到一株对番茄细菌性叶斑病拮抗能力较强的菌株264ZY7,其抑菌圈直径为1.22cm。264ZY7菌体短杆状,大小为1.534μm×0.571μm~3.210μm×0.781μm,革兰氏阳性菌,根据形态特征,并结合16SrDNA和gyrB基因序列相似性分析,将菌株264ZY7鉴定为解淀粉芽孢杆菌Bacillusamyloliquefaciens。通过室内盆栽防效试验,结果表明该菌株对番茄细菌性叶斑病的防效达69%,此外,该菌株对8种病原真菌具有抑菌能力,说明抑菌谱较宽,且抑菌率均大于30%,对番瓜根腐病菌(Fusariumsp.)效果最明显,抑菌率为52.84%。该研究结果为高寒草地紫花针茅内生细菌264ZY47的进一步开发利用提供了依据。

 References

[1]  Huang C J, Zhao S Y, Wang J C, et al . Photosynthetic characteristics and yield of potato in potato/maize intercropping systems with different row number ratios. Chinese Journal of Eco-Agriculture, 2012, 20(11): 1443-1450.
[2]  Yi Y J, Luo K, Liu E M. Effect of endophytic bacterial on biological control of plant disease. Journal of Nuclear Agricultural Sciences, 2007, 21(5): 474-477.
[3]  Huang C J, Zhao S Y, Wang C L, et al . Effect of potato/maize intercropping on photosynthetic characteristics and yield in two potato varieties. Acta Agronomica Sinica, 2013, 39(2): 330-342.
[4]  Li C S. A review on research progress of the biologicial control of soil-borne plant diseases with bacteria. Chinese Journal of Biological Control, 1992, 8(4): 168-172.
[5]  Neumann A, Schmidtke K, Rauber R. Effects of crop density and tillage system on grain yield and N uptake from soil and atmosphere of sole and intercropped pea and oat. Field Crops Research, 2007, 100: 285-293.
[6]  Luo M Y, Lu X J, Liao X L. Research advances on application of endophytic bacteria. Modern Agricultural Science and Technology, 2010, (7): 11-12.
[7]  Chen G, Guo L M, Ren C Z, et al . Effects of two row spaces and intercropping on forage and crude protein yields of oat ( Avena sativa L.) and common vetch ( Vicia sativa L.). Acta Agronomica Sinica, 2011, 37(11): 2066-2074.
[8]  Huang X, Xu L L, Huang R S, et al . Research advance in controlling plant diseases by Bacillus subtilis . Biotechnology Bulletin, 2010, (1): 24-28.
[9]  Fang Z D. Research Method of Plant Pathology (Third Edition)[M]. Beijing: Chinese Agriculture Press, 1998: 110, 182-183.
[10]  Arnon D T. Copper enzyme in isolated chloroplasts polyphenoloxidase in Beta vulgaris . Plant Physiology, 1949, 24(1): 1-15.
[11]  Gao X X, Man B Y, Chen X R, et al . Identification and determination of biological characteristics of Kobresia capillifolia endophytic bacteria X4 in the East Qilian Mountain Alpine grasslands. Acta Prataculturae Sinica, 2013, 22(4): 137-146.
[12]  Chen Y X, Chen X H, Tang Y Q, et al . Effect of nitrogen fertilizer on dry matter accumulation and yield in wheat/maize/soybean intercropping systems. Acta Prataculturae Sinica, 2014, 23(1): 73-83.
[13]  Zhao J, Lan C Z, Chen Q H, et al . Isolation of endophytic bacteria from tomato and selection of antagonistic bacteria to tomato bacterial wilt disease. Wuyi Science Journal, 2006, 22(1): 49-54.
[14]  Tang X M, Zhong R C, Jie H K, et al . Effect of shading on photosynthesis and chlorophyll fluorescence characteristic of peanut under different inter-row space in cassava-peanut intercropping. Southwest China Journal of Agricultural Sciences, 2011, 24(5): 1703-1707.
[15]  Li Z D, Chen X R, Yang C D. Identification of Polygonum viviparum endophytic bacteria Z17 and its capacity to antagonistic towards pathogenic fungi. Pratacultural Science, 2011, 28(12): 2096-2101.
[16]  Li Z, Qin X Y, Wang X G, et al . Effect of intercropping with maize on photosynthesis and chlorophyll fluorescence parameters of soybean. Soybean Science, 2010, 29(5): 808-811.
[17]  Wang L, Lee F, Tai C, et al . Comparison of gyrB gene sequences,16S rRNA gene sequences and DNA-DNA hybridization in the Bacillus subtilis group. International Journal of Systematic and Evolutionary Microbiology, 2007, 57: 1846-1850.
[18]  Chen Y, Zou C Y. A study on optimum structure disposition of intercroppings of maize-soybean complex and its productivity. Resources Science, 1999, 21(4): 75-79.
[19]  Yamamoto S, Harayama S. PCR amplification and direct sequencing of gyrB genes with universal primers and their application to the detection and taxonomic analysis of Pseudomonas putida strains. Applied Environmental Microbiology, 1995, 61: 1104-1109.
[20]  Chen Y X, Zhou D W, Zhang Y F. Yield and photosynthesis of intercropped maize and alfalfa. Chinese Journal of Grassland, 2004, 12(2): 107-112.
[21]  He H, Cai X Q, Hong Y C, et al . Selection of endophytic antifungal bacteria from capsicum. Chinese Journal of Biological Control, 2002, 18(4): 171-175.
[22]  Gong X J, Teng Y F, Qian C R, et al . Physiological effect of maize/chili pepper intercroppingⅠ.The influence of different intercropping patterns on photosynthetic rate and yield of maize/chili pepper. Chinese Agricultural Science, 2010, 26(21): 111-114.
[23]  Li Q Q, Luo K, Lin W, et al . Isolation of tomato endophytic antagonists against Ralstonia solanacearum . Acta Phytopathologica Sinica, 2003, 33(4): 364-367.
[24]  Zhang J Z, Zhang Q Y, Sun G F, et al . Effects of drought stress and re-watering on growth and photosynthesis of Hosta . Acta Prataculturae Sinica, 2014, 23(1): 167-176.
[25]  Huang M Y, Gu W J, Zhang F B, et al . Identification and fermentation of antagonistic bacterium against Ralstonia solanacearum . Microbiology, 2011, 38(2): 214-220.
[26]  Xu D Q. Some problems in stomatal limitation analysis of photosynthesis. Plant Physiology Communications, 1997, 33(4): 241-244.
[27]  Li S M. Mechanism of Interspecific Facilitation on Phosphorus Uptake by Crops in Intercropping Systems[D]. Beijing: China Agricultural University, 2003.
[28]  Li W J, Jiang R B. Isolation of tomato antagonists against Ralstonia solanacearum . Journal of Microbiology, 2007, 27(1): 5-8.
[29]  Zhang E H, Li L L, Huang G B, et al . Regulation of fertilizes application on yield and root growth of spring wheat faba bean intercropping system. Chinese Journal of Applied Ecology, 2002, 13(8): 939-942.
[30]  Ye Y L. Effect of Intercropping on Nitrogen and Water Use[D]. Beijing: China Agricultural University, 2003.
[31]  汪春明. 马铃薯连作栽培与间作调控对根际土壤的影响[D]. 银川: 宁夏大学, 2014.
[32]  杜燕萍, 马均伊, 常克勤, 等. 宁夏南部山区裸燕麦丰产栽培技术. 内蒙古农业科技, 2008, (1): 103-104.
[33]  吴娜, 胡跃高, 任长忠, 等. 两种灌溉方式下保水剂用量对春播裸燕麦土壤氮素的影响. 草业学报, 2014, 23(2): 346-351.
[34]  贺佳, 安瞳昕, 韩学坤, 等. 间作群体生态生理研究进展. 作物杂志, 2011, 4: 7-11.
[35]  高阳, 段爱旺, 刘祖贵, 等. 玉米和大豆条带间作模式下的光环境特性. 应用生态学报, 2008, 19(6): 1248-1254.
[36]  刘忠宽, 曹卫东, 秦文利, 等. 玉米-紫花苜蓿间作模式与效应研究. 草业学报, 2009, 18(6): 158-163. 浏览
[37]  Yang M L, Sun X L. Research of endophytic bacteria. Microbiology, 1998, 25(4): 224-227.
[38]  肖继坪, 颉炜清, 郭华春. 马铃薯与玉米间作群体的光合及产量效应. 中国马铃薯, 2011, 25(6): 339-341.
[39]  黄承建, 赵思毅, 王季春, 等. 马铃薯/玉米不同行数比套作对马铃薯光合特性和产量的影响. 中国生态农业学报, 2012, 20(11): 1443-1450.
[40]  黄承建, 赵思毅, 王昌龙, 等. 马铃薯/玉米套作对马铃薯品种光合特性及产量的影响. 作物学报, 2013, 39(2): 330-342.
[41]  陈恭, 郭丽梅, 任长忠, 等. 行距及间作对箭筈豌豆与燕麦青干草产量和品质的影响. 作物学报, 2011, 37(11): 2066-2074.
[42]  张恩和, 李玲玲, 黄高宝, 等. 供肥对小麦间作蚕豆群体产量及根系的调控.应用生态学报, 2002, 13(8): 939-942.
[43]  叶优良. 间作对氮素和水分利用的影响[D]. 北京: 中国农业大学, 2003.
[44]  Zhao H, Lu C T, Liu H Y, et al . Identification of a Bacillus amyloliquefaciens strain and preliminary research on its antifungal activity. Journal of Henan Agricultural Sciences, 2014, 43(4): 75-79.
[45]  Gao Y, Duan A W, Liu Z G, et al . Light environment characteristics in maize_soybean strip intercropping system. Chinese Journal of Applied Ecology, 2008, 19(6): 1248-1254.
[46]  Zhao T C, Yu L, Sun F Z, et al . Tomato bacterial speck disease and its prevention. Plant Protection, 1999, 25(4): 56.
[47]  Liu Z K, Cao W D, Qin W L, et al . A study on the pattern and effect of Zea mays intercropping with Medicago sativa . Acta Prataculturae Sinica, 2009, 18(6): 158-163.
[48]  Yunis G P, Crall J M. First report of a bacreial speck of tomato by Pseudomonas syringa pv. tomato in Georgia. Plant Disease, 1980, 83: 199.
[49]  Song Y N, Zhang F S, Marschner P, et al . Community composition of ammonia-oxidizing bacteria in the rhizosphere of intercropped wheat ( Triticum aestivum L.), maize ( Zea mays L.), and faba bean ( Vicia faba L.). Biology and Fertility of Soils, 2007, 44: 307-314.
[50]  Bonn, Martin H L. Bacterial spot of tomato leaves occurrence law in Frolidia. Journal of Experimental Agriculture, 1980, 39: 479-485.
[51]  Henrik H N, Bjarne J, Julia K G, et al . Legume-cereal intercropping: the practical application of diversity, competition and facilitation in arable and organic cropping systems. Renewable Agriculture and Food Systems, 2007, 23(1): 3-12.
[52]  Zhao T C, Sun F Z, Song W S. Pathogen identification of bacterial speck of tomato. Acta Phytopathologica Sinica, 2001, 31(1): 37-42.
[53]  Xiao J P, Xie W Q, Guo H C. Characteristics of photosynthesis and yield in potato intercropping with maize. Chinese Potato Journal, 2011, 25(6): 339-341.
[54]  Deng G, Qu X, Chen X R, et al . Pathogen identification of bacterial leaf speck of tomato based on 16S rDNA, physiology and biochemistry in Gansu. Plant Protection, 2008, 34(5): 47-51.
[55]  陈远学, 陈晓辉, 唐义琴, 等. 不同氮用量下小麦/玉米/大豆周年体系的干物质积累和产量变化. 草业学报, 2014, 23(1): 73-83. 浏览
[56]  Zhang J, Ran X X, Zhu T H, et al . Bacillus amyloliquefaciens antimicrobial effect on Pestalotiopsis guepini (Desm.) Stey. Journal of Northeast Forestry University, 2014, (7): 122-125.
[57]  Chen S Y, Yang B Y, Gao M Y, et al . Inhibition of sclerotia formation of sclerotinia sclerotiorum by Bacillus amyloliquefaciens . Chinese Journal of Applied & Environmental Biology, 2005, 11(3): 373-376.
[58]  唐秀梅, 钟瑞春, 揭红科, 等. 间作遮荫对花生光合作用及叶绿素荧光特性的影响. 西南农业学报, 2011, 24(5): 1703-1707.
[59]  李植, 秦向阳, 王晓光, 等. 大豆/玉米间作对大豆叶片光合特性和叶绿素荧光动力学参数的影响. 大豆科学, 2010, 29(5): 808-811.
[60]  Quan C S, Wang J H, Xu H T, et al . Identification and characterization of a Bacillus amyloliquefaciens with high antifungal activity. Acta Microbiologica Sinica, 2006, 46(1): 7-12.
[61]  陈颖, 邹超亚. 玉米大豆间作复合群体优化配置与生产力研究. 资源科学, 1999, 21(4): 75-79.
[62]  陈玉香, 周道玮, 张玉芬. 玉米、苜蓿间作的产草量及光合作用. 草地学报, 2004, 12(2): 107-112.
[63]  宫秀杰, 滕云飞, 钱春荣, 等. 玉米/辣椒间作复合群体生理效应研究Ⅰ不同间作方式对玉米/辣椒光合速率和产量的影响.中国农学通报, 2010, 26(21): 111-114.
[64]  张金政, 张起源, 孙国峰, 等. 干旱胁迫及复水对玉簪生长和光合作用的影响. 草业学报, 2014, 23(1): 167-176. 浏览
[65]  许大全. 光合作用气孔限制分析中的一些问题. 植物生理学通讯, 1997, 33(4): 241-244.
[66]  李淑敏. 间作作为吸收磷的种间促进作用机制研究[D]. 北京: 中国农业大学, 2003.
[67]  Zhao X Y, Yang H, Chen J G, et al . Screening and fermentation optimization of antagonistic bacillus against tobacco bacterial wilt. Hubei Agricultural Sciences, 2014, (8): 1810-1814.
[68]  Fan X J, Yang C Q, Qiu S X, et al . Identification of biocontrol bacterial strain and its antagonistic activity to tomato bacterial speck and colonizing ability in tomato. Journal of Fujian Agriculture and Forestry University (Natural Science Edition), 2013, 42(4): 337-341.
[69]  赵廷昌, 于莉, 孙福在, 等. 番茄细菌性斑点病及其防治. 植物保护, 1999, 25(4): 56.
[70]  赵廷昌, 孙福在, 宋文生. 番茄细菌性斑点病病原菌鉴定. 植物病理学报, 2001, 31(1): 37-42.
[71]  邓刚, 屈星, 陈秀蓉, 等. 甘肃番茄细菌性斑点病病原菌鉴定. 植物保护, 2008, 34(5): 47-51.
[72]  易有金, 罗宽, 刘二明. 内生细菌在植物病害生物防治中的作用. 核农学报, 2007, 21(5): 474-477.
[73]  李长松. 拮抗性细菌生物防治植物土传病害的研究进展. 生物防治通报, 1992, 8(4): 168-172.
[74]  罗茗月, 路雪君, 廖晓兰. 植物内生细菌的应用研究进展. 现代农业科技, 2010, (7): 11-12.
[75]  黄曦, 许兰兰, 黄荣韶,等. 枯草芽孢杆菌在抑制植物病原菌中的研究进展. 生物技术通报, 2010, (1): 24-28.
[76]  方中达. 植病研究方法(第三版)[M]. 北京: 中国农业出版社, 1998: 110, 182-183.
[77]  高晓星, 满百膺, 陈秀蓉, 等. 东祁连山线叶嵩草内生细菌X4的产吲哚乙酸、解磷、抗菌和耐盐特性研究及分子鉴定. 草业学报, 2013, 22(4): 137-146. 浏览
[78]  赵健, 兰成忠, 陈庆河, 等. 抗番茄青枯病内生拮抗细菌的筛选及室内防效测定. 武夷科学, 2006, 22(1): 49-54.
[79]  李振东, 陈秀蓉, 杨成德. 珠芽蓼内生菌Z17抑菌能力测定及其鉴定. 草业科学, 2011, 28(12): 2096-2101.
[80]  何红, 蔡学清, 洪永聪, 等. 辣椒内生细菌的分离及拮抗菌的筛选. 中国生物防治, 2002, 18(4): 171-175.
[81]  黎起秦, 罗宽, 林纬, 等. 番茄青枯病内生拮抗细菌的筛选. 植物病理学报, 2003, 33(4): 364-367.
[82]  黄明媛, 顾文杰, 张发宝, 等. 番茄青枯病拮抗菌筛选鉴定及其发酵条件初探. 微生物学通报, 2011, 38(2): 214-220.
[83]  李伟杰, 姜瑞波. 番茄青枯病拮抗菌的筛选. 微生物学杂志, 2007, 27(1): 5-8.
[84]  杨梅莲, 孙晓璐. 植物内生细菌的研究. 微生物学通报, 1998, 25(4): 224-227.
[85]  张静, 冉晓潇, 朱天辉, 等. 解淀粉芽孢杆菌对山茶灰斑病菌的抑制作用. 东北林业大学学报, 2014, (7): 122-125.
[86]  陈士云, 杨宝玉, 高梅影, 等. 一株抑制油菜核盘菌菌核形成的解淀粉芽孢杆菌. 应用与环境生物学报, 2005, 11(3): 373-376.
[87]  权春善, 王军华, 徐洪涛, 等. 一株抗真菌解淀粉芽孢杆菌的分离鉴定及其发酵条件的初步研究. 微生物学报, 2006, 46(1): 7-12.
[88]  赵辉, 鲁传涛, 刘红彦, 等. 一株生防芽孢杆菌的鉴定及其抑菌活性的初步研究. 河南农业科学, 2014, 43(4): 75-79.
[89]  赵晓燕, 杨欢, 陈建刚, 等. 一株烟草青枯病生防菌的筛选鉴定及其发酵优化. 湖北农业科学, 2014, (8): 1810-1814.
[90]  范晓静, 杨春泉, 邱思鑫, 等.番茄细菌性斑点病生防菌的鉴定、防病及定殖力. 福建农林大学学报(自然科学版), 2013, 42(4): 337-341.
[91]  ||

Full-Text

Contact Us

service@oalib.com

QQ:3279437679

WhatsApp +8615387084133